子痫前期中胎盘组织中 SHH 的表达与滋养细胞氧化应激损伤。
SHH expression in placental tissues and trophoblast cell oxidative stress injury during preeclampsia.
机构信息
Department of Gynaecology and Obstetrics, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, P.R. China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Jul;23(14):6026-6034. doi: 10.26355/eurrev_201907_18415.
OBJECTIVE
To assess the association between Sonic hedgehog (SHH) protein expression levels on placental tissues and the presence of preeclampsia and to assess oxidative stress in cultured trophoblast cells from placentas of women with preeclampsia treated under different conditions.
PATIENTS AND METHODS
We enrolled 30 pregnant women with early-onset preeclampsia (<34 weeks), 30 with late-onset preeclampsia (≥34 weeks), and 60 healthy pregnant women with early or late gestational ages for two control groups. We used ELISA tests to measure individual serum SHH levels. Also, we used real-time PCR, and Western blotting to detect Shh transcript and protein levels in placental tissues, respectively, and immunohistochemistry to localize the protein in placental tissues. We isolated trophoblast cells from placentas and cultured them in vitro under different conditions to compare oxidative stress injury and apoptosis rates between them.
RESULTS
The mean serum SHH levels in early and late preeclampsia groups were similar (p>0.05). Placentas of women with and without preeclampsia both expressed SHH protein mainly in syncytiotrophoblast cytoplasms. The mean placental SHH protein expressions in both women with early-onset and late-onset preeclampsia were significantly higher than that in the women of the late gestational age control group (p<0.05). The mean Shh mRNA abundances in early-onset and late-onset preeclampsia placentas were significantly higher than that in the late gestational age control placentas (p<0.05). The placental mitochondria oxidative stress injury was significantly higher in the preeclampsia than in the control group (p<0.05). The oxidative stress injury and apoptosis of trophoblast cells were higher in preeclampsia groups than in the late gestational age control group (p<0.05). Trophoblast cells treated with SHH serum had significantly lower oxidative stress than the same cells grown in the presence of preeclamptic serum (p<0.05).
CONCLUSIONS
The expression of Shh in placental tissues is associated with the oxidative stress mechanism during preeclampsia.
目的
评估胎盘组织中 Sonic hedgehog（SHH）蛋白表达水平与子痫前期的关系，并评估不同条件下子痫前期患者胎盘滋养细胞培养物中的氧化应激。
方法
我们招募了 30 名早发型子痫前期（<34 周）、30 名晚发型子痫前期（≥34 周）和 60 名早、晚孕期健康孕妇作为两个对照组。我们使用 ELISA 试验测量个体血清 SHH 水平。还使用实时 PCR 和 Western blot 分别检测胎盘组织中的 Shh 转录物和蛋白水平，并使用免疫组织化学法定位胎盘组织中的蛋白。我们从胎盘分离滋养细胞并在体外进行不同条件下的培养，以比较它们之间的氧化应激损伤和凋亡率。
结果
早发型和晚发型子痫前期组的平均血清 SHH 水平相似（p>0.05）。有子痫前期和无子痫前期的胎盘均主要在合体滋养细胞质中表达 SHH 蛋白。早发型和晚发型子痫前期胎盘的平均 SHH 蛋白表达均明显高于晚孕期对照组（p<0.05）。早发型和晚发型子痫前期胎盘的 Shh mRNA 丰度明显高于晚孕期对照组（p<0.05）。子痫前期组胎盘线粒体氧化应激损伤明显高于对照组（p<0.05）。子痫前期组滋养细胞的氧化应激损伤和凋亡均高于晚孕期对照组（p<0.05）。与在子痫前期血清存在的情况下生长的相同细胞相比，用 SHH 血清处理的滋养细胞的氧化应激损伤显著降低（p<0.05）。
结论
胎盘组织中 Shh 的表达与子痫前期期间的氧化应激机制有关。
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