Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China.
Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
Phytochem Anal. 2020 Mar;31(2):164-182. doi: 10.1002/pca.2876. Epub 2019 Jul 31.
Dioscorea species, which contain abundant steroidal saponins, have been used as folk medicines or raw materials to synthesise steroid drugs.
To establish a rapid chemotaxonomic method that will comprehensively resolve confusions about genetic relationships of genus Dioscorea.
A comprehensive strategy using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) was firstly proposed to evaluate the chemotaxonomy of 12 species (27 taxa) from China by hierarchical cluster analysis (HCA) based on the variations of the identified metabolites.
Twenty-eight secondary metabolites (mainly steroidal saponins) were identified. The MS fragmentation patterns of DA (a new acetylated steroidal saponin at C-7 position) were firstly reported. Moreover, eight major steroidal saponins were further quantified simultaneously by UPLC-QTOF-MS method. According to HCA results, D. bulbifera L. was distinguished with species of sect. Stenophora Uline for pennogenin-type steroidal saponins. Dioscorea zingiberensis exhibited far distance from other members of sect. Stenophora Uline for two unique saponins. Dioscorea banzhuana may be reclassified into sect. Stenophora. Dioscorea nipponica subsp. rosthornii and D. collettii var. hypoglauca might be separated from their original subspecies/varieties as new species, respectively.
The chemotaxonomic method was successfully applied in the study of genetic relationships of Dioscorea species. This study not only enhanced the understanding of chemical constituents, but also laid basic theoretical foundations for the rational utilisation and chemotaxonomy of genus Dioscorea.
含丰富甾体皂苷的薯蓣属植物被广泛用作民间药物或甾体药物合成的原料。
建立一种快速的化学分类方法，全面解决薯蓣属植物遗传关系的混乱。
首次提出了一种综合策略，即利用超高效液相色谱-四级杆飞行时间串联质谱（UHPLC-QTOF-MS/MS），通过基于鉴定代谢物变化的层次聚类分析（HCA）来评估来自中国的 12 个种（27 个分类群）的化学分类学。
鉴定出 28 种次生代谢物（主要为甾体皂苷）。首次报道了 DA（C-7 位乙酰化甾体皂苷）的 MS 裂解模式。此外，还通过 UPLC-QTOF-MS 方法进一步同时定量了 8 种主要甾体皂苷。根据 HCA 结果，D. bulbifera L. 与 sect. stenophora Uline 的种以沿阶草型甾体皂苷区分。Dioscorea zingiberensis 因其两种独特的皂苷而与 sect. stenophora Uline 的其他成员相距甚远。Dioscorea banzhuana 可能被重新归类为 sect. stenophora。Dioscorea nipponica subsp. rosthornii 和 D. collettii var. hypoglauca 可能分别作为新种从其原始亚种/变种中分离出来。
化学分类方法成功应用于薯蓣属植物遗传关系的研究。本研究不仅加深了对化学成分的认识，而且为薯蓣属植物的合理利用和化学分类奠定了基础理论基础。
