Chao Y S, Ding X H, Dai P H, Wu T J, Pan T C, Hao Q L, Yamin T T
Merck Institute for Therapeutic Research, Merck, Sharpe and Dohme Research Laboratories, Rahway, NJ 07065-0900.
Nucleic Acids Res. 1988 Jul 25;16(14B):7061-70. doi: 10.1093/nar/16.14.7061.
In order to study the expression of the apolipoprotein (apo) A-I gene, we have isolated and characterized the structural gene encoding rat apo A-I. The 5' flanking sequence of the apo A-I gene was placed upstream of the coding sequence of the bacterial chloramphenicol acetyl transferase (CAT) gene, such that the expression of CAT activity in cultured cells is under the control of the promoter and regulatory sequences of the rat apo A-I gene. By transient transfection, nucleotide deletion and substitution methods, it was demonstrated that the nucleotide sequences between -464 and -148 upstream from the start of transcription of the rat apo A-I gene are required for the expression of this gene in Hep G2 cells and that these sequences function with an enhancer-like activity.
为了研究载脂蛋白（apo）A-I基因的表达，我们分离并鉴定了编码大鼠apo A-I的结构基因。将apo A-I基因的5'侧翼序列置于细菌氯霉素乙酰转移酶（CAT）基因编码序列的上游，使得培养细胞中CAT活性的表达受大鼠apo A-I基因启动子和调控序列的控制。通过瞬时转染、核苷酸缺失和取代方法，证明大鼠apo A-I基因转录起始点上游-464至-148之间的核苷酸序列是该基因在Hep G2细胞中表达所必需的，并且这些序列具有类似增强子的活性。
