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转录因子LF-A1与几个肝脏特异性基因启动子区域中的一个双组分识别序列相互作用。

The transcription factor LF-A1 interacts with a bipartite recognition sequence in the promoter regions of several liver-specific genes.

作者信息

Ramji D P, Tadros M H, Hardon E M, Cortese R

机构信息

European Molecular Biology Laboratory, Heidelberg, FRG.

出版信息

Nucleic Acids Res. 1991 Mar 11;19(5):1139-46. doi: 10.1093/nar/19.5.1139.

DOI:10.1093/nar/19.5.1139
PMID:2020548
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC333792/
Abstract

The transcription factors LF-A1 and LF-B1 are required for the cell-specific expression of the human alpha 1-antitrypsin gene in hepatocytes. We report here the purification and preliminary characterization of LF-A1. This protein, purified to homogeneity from calf liver nuclei by site-specific DNA affinity chromatography and reverse-phase HPLC, has a molecular mass of 40 kDa. Binding sites of LF-A1 are present in the promoter regions of several genes expressed in the liver (alpha 1-antitrypsin, apolipoproteins A1, B1, A4 and pyruvate kinase). Interestingly, the binding site of LF-A1 is bipartite and consists of two short sequence motifs (consensus: TGGACT/CT/C and TGGCCC) separated by a variable 'spacer' region. Insertion or deletion of 1-4 nucleotides in the 'spacer' region of the site in the alpha 1-antitrypsin promoter does not abolish DNA binding.

摘要

转录因子LF-A1和LF-B1是人类α1-抗胰蛋白酶基因在肝细胞中细胞特异性表达所必需的。我们在此报告LF-A1的纯化及初步特性。该蛋白通过位点特异性DNA亲和层析和反相高效液相色谱从小牛肝细胞核中纯化至同质，分子量为40 kDa。LF-A1的结合位点存在于肝脏中表达的几个基因（α1-抗胰蛋白酶、载脂蛋白A1、B1、A4和丙酮酸激酶）的启动子区域。有趣的是，LF-A1的结合位点是二分的，由两个短序列基序（共有序列：TGGACT/CT/C和TGGCCC）组成，中间被一个可变的“间隔”区域隔开。在α1-抗胰蛋白酶启动子位点的“间隔”区域插入或缺失1-4个核苷酸不会消除DNA结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/42f2b7e0782e/nar00241-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/6597621de9e4/nar00241-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/1d9b37069a62/nar00241-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/92722291f76a/nar00241-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/7f2b469347f5/nar00241-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/83acbb7e9a20/nar00241-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/42f2b7e0782e/nar00241-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/6597621de9e4/nar00241-0161-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/1d9b37069a62/nar00241-0161-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/92722291f76a/nar00241-0162-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/7f2b469347f5/nar00241-0162-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/83acbb7e9a20/nar00241-0162-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed1c/333792/42f2b7e0782e/nar00241-0164-a.jpg

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