Department of Urology Surgery, Tengzhou Central People's Hospital, Tengzhou, Zhaozhuang, Shandong 277500, P.R. China.
Oncol Rep. 2019 Oct;42(4):1431-1440. doi: 10.3892/or.2019.7246. Epub 2019 Jul 23.
Jumonji domain‑containing protein 2A (JMJD2A) has been identified to promote cell proliferation in bladder cancer; however, it remains undetermined whether JMJD2A regulates cell migration and invasion in bladder cancer. The aim of the present study was to further investigate the roles of JMJD2A in bladder cancer. The expression levels of JMJD2A in bladder cancer tissues and cell lines were established by RT‑qPCR assays and western blot analysis. Moreover, by gain‑ and loss‑of‑function assays, the effects of JMJD2A on migration and invasion as well as proliferation were investigated in bladder cancer cells. The results revealed that the expression level of JMJD2A was significantly upregulated in bladder cancer tissues and cell lines compared to adjacent non‑tumor tissues and a human immortalized bladder urothelial cell line. Kaplan‑Meier survival analysis indicated that patients with high JMJD2A expression level had shorter overall survival. Moreover, JMJD2A could promote cell migration and invasion by facilitating epithelial‑mesenchymal transition (EMT) in bladder cancer. In addition, it was determined that JMJD2A promoted EMT through regulation of SLUG expression. Collectively, our findings revealed that JMJD2A may act as an oncogene and participate in bladder cancer progression, which provides a promising therapeutic strategy for patients with bladder cancer.
Jumonji 结构域包含蛋白 2A（JMJD2A）已被鉴定可促进膀胱癌中的细胞增殖；然而，JMJD2A 是否调节膀胱癌中的细胞迁移和侵袭仍未确定。本研究旨在进一步探讨 JMJD2A 在膀胱癌中的作用。通过 RT-qPCR 检测和 Western blot 分析，建立了膀胱癌组织和细胞系中 JMJD2A 的表达水平。此外，通过增益和失活功能测定，研究了 JMJD2A 对膀胱癌细胞迁移、侵袭和增殖的影响。结果显示，与相邻非肿瘤组织和人永生化膀胱尿路上皮细胞系相比，膀胱癌组织和细胞系中 JMJD2A 的表达水平显著上调。Kaplan-Meier 生存分析表明，JMJD2A 高表达的患者总生存期较短。此外，JMJD2A 可通过促进上皮间质转化（EMT）促进膀胱癌细胞的迁移和侵袭。此外，还确定 JMJD2A 通过调节 SLUG 表达促进 EMT。综上所述，我们的研究结果表明，JMJD2A 可能作为一种癌基因参与膀胱癌的进展，为膀胱癌患者提供了一种有前途的治疗策略。
