EB 病毒感染对免疫相关性全血细胞减少症患者 CD19+B 淋巴细胞的影响。
Effects of Epstein-Barr Virus Infection on CD19+ B Lymphocytes in Patients with Immunorelated Pancytopenia.
机构信息
Department of Hematology, Tianjin Medical University General Hospital, China.
出版信息
J Immunol Res. 2020 Feb 17;2020:4098235. doi: 10.1155/2020/4098235. eCollection 2020.
OBJECTIVES
To explore effects of Epstein-Barr virus (EBV) infection on CD19+ B lymphocytes in patients with immunorelated pancytopenia (IRP).
METHODS
An enzyme-linked immunosorbent assay (ELISA) in vitro diagnostic kit was used to detect EBV capsid antigen- (CA-) IgG and VCA-IgM antibodies in the serum. We analyzed the EBV-DNA copies of CD19+ B lymphocyte by using real-time quantitative polymerase chain reaction (RT-qPCR). CD21, CD23, CD5, CD80, and CD86 receptors on the surfaces of CD19+ B cells were detected by flow cytometry (FCM). The correlation between these receptors and EBV-DNA copies were evaluated.
RESULTS
The results revealed that the positive rate of EBVCA-IgM and CD19+ B lymphocyte EBV-DNA copy in the IRP group were significantly higher than those in the control group ( < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects ( < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects ( < 0.05). CD19+ B lymphocyte EBV-DNA copies were also more abundant in IRP patients than in control subjects (.
CONCLUSIONS
EBV infection may activate CD19+ B lymphocytes and further disrupt bone marrow hematopoiesis in IRP patients.
目的
探讨 EBV 感染对免疫相关性全血细胞减少症（IRP）患者 CD19+B 淋巴细胞的影响。
方法
采用酶联免疫吸附试验（ELISA）体外诊断试剂盒检测血清 EBV 衣壳抗原（CA）-IgG 和 VCA-IgM 抗体，实时荧光定量聚合酶链反应（RT-qPCR）检测 CD19+B 淋巴细胞 EBV-DNA 拷贝数。采用流式细胞术（FCM）检测 CD19+B 细胞表面 CD21、CD23、CD5、CD80 和 CD86 受体，并评估这些受体与 EBV-DNA 拷贝数之间的相关性。
结果
结果显示，IRP 组 EBVCA-IgM 和 CD19+B 淋巴细胞 EBV-DNA 拷贝的阳性率明显高于对照组（<0.05）。IRP 患者的 CD19+B 淋巴细胞 EBV-DNA 拷贝数也明显高于对照组（<0.05）。
结论
EBV 感染可能激活 CD19+B 淋巴细胞，进一步破坏 IRP 患者骨髓造血。
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