Akbulut Aylin, Keseroglu Bugra Bilge, Koca Gokhan, Yuceturk Cem Nedim, Ozgur Berat Cem, Surer Hatice, Ogus Elmas, Yumuşak Nihat, Karakaya Jale, Korkmaz Meliha
Departments of Nuclear Medicine.
Urology.
Nucl Med Commun. 2019 Oct;40(10):1011-1021. doi: 10.1097/MNM.0000000000001070.
Renal ischemia-reperfusion injury (RIRI) may occur secondary to several reasons leading to renal failure. Coenzyme-Q10 (CoQ10) is a well-known antioxidant. However, the effects CoQ10 against RIRI have not been evaluated. Our aim was to evaluate protective effects of CoQ10 to renal ischemia-reperfusion by biochemical, immunohistochemical and scintigraphic findings.
Thirty Wistar-albino rats were randomly separated into groups of 10; Group Sham; Group ischemia-reperfusion (IR) had left renal pedicle clamping; Group CoQ10+IR had IR and CoQ10. Twenty-four hours later after reperfusion, scintigraphy was performed and after that, rats were sacrificed. To demonstrate effects of RIRI, serum urea and creatinine levels and tissue levels oxidative stress markers were evaluated. Both kidneys were subjected to histopathological evaluation and to confirm RIRI-induced immunohistochemical aspects of apoptosis, terminal-deoxynucleotidyl-transferase mediated-deoxyuridine-triphosphate-nick-end-labeling assay and caspase-3 were assessed.
Tissue oxidative stress, histopathologic changes, apoptosis scores and quantitative scintigraphic parameters were significantly higher in Group IR compared with Group Sham. Although tissue oxidative stress levels and histopathologic changes were not significant, quantitative scintigraphic parameters of contralateral kidney of Group IR were significantly increased. Compared with Group IR, Group CoQ10+IR presented decreased tissue oxidative stress levels; decreased scores of histopathology and apoptosis; and decreased quantitative scintigraphic parameters with increased split renal function in ischemic kidney.
Our results suggest that other than its antioxidant properties, CoQ10 shows antiperoxidative, antiapoptotic and antiinflammatory potential in protecting renal functioning of ischemic kidney. Furthermore, our results show that renal scintigraphy is a feasible method to detect early changes in renal functioning after RIRI.
肾缺血再灌注损伤（RIRI）可能继发于多种导致肾衰竭的原因。辅酶Q10（CoQ10）是一种著名的抗氧化剂。然而，CoQ10对RIRI的作用尚未得到评估。我们的目的是通过生化、免疫组织化学和闪烁扫描结果评估CoQ10对肾缺血再灌注的保护作用。
30只Wistar白化大鼠随机分为10只一组；假手术组；缺血再灌注（IR）组进行左肾蒂夹闭；CoQ10+IR组进行IR并给予CoQ10。再灌注24小时后进行闪烁扫描，之后处死大鼠。为了证明RIRI的作用，评估血清尿素和肌酐水平以及组织氧化应激标志物水平。对双侧肾脏进行组织病理学评估，并通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记法和半胱天冬酶-3评估来确认RIRI诱导的凋亡免疫组织化学方面。
与假手术组相比，IR组的组织氧化应激、组织病理学变化、凋亡评分和定量闪烁扫描参数显著更高。虽然组织氧化应激水平和组织病理学变化不显著，但IR组对侧肾脏的定量闪烁扫描参数显著增加。与IR组相比，CoQ10+IR组的组织氧化应激水平降低；组织病理学和凋亡评分降低；定量闪烁扫描参数降低，缺血肾脏的分肾功能增加。
我们的结果表明，CoQ10除了具有抗氧化特性外，在保护缺血肾脏的肾功能方面还具有抗过氧化、抗凋亡和抗炎潜力。此外，我们的结果表明，肾闪烁扫描是检测RIRI后肾功能早期变化的一种可行方法。
