Wang Y N, Meng X C, Wang C S E, Yang C, Qian J M, Li J N
Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Beijing Macro-Union Pharmaceutical Limited Corporation, Beijing 101116, China.
Zhonghua Nei Ke Za Zhi. 2019 Aug 1;58(8):584-591. doi: 10.3760/cma.j.issn.0578-1426.2019.08.007.
To investigate the effects of probiotics and synbiotics on inflammation and microbiota of acute colitis in mice. C57BL/6J mice were divided into 4 groups randomly. Each group had 10 mice and was given 2.5% dextran sulfate sodium (DSS) drinking water for 5 days other than the blank control group. Except for model control group, other two groups were administrated with probiotics and synbiotics, respectively. Probiotics was composed of , while synbiotics was composed of the aforementioned probiotics, inulin and galactooligosaccharide. Feces of different periods and mucosa samples were collected to analyze the differences of enteric flora by 16s rDNA sequencing. (1) Pathological scores in probiotics group and synbiotics group were 5.40±2.79 and 7.25±2.87, respectively, which were significantly lower than those in the model control group with scores 27.00±7.94. Model control group, probiotics group and synbiotics group showed lower flora diversity, increased and decreased than blank control group. The mucosal microbiota was different from fecal flora in abundance and species for each group, and was more common in mucosa. Probiotics and synbiotics alleviate the inflammation of acute colitis in mice. Imbalance of beneficial genera to harmful genera is the characteristic of acute colitis. Supplementation of probiotics and synbiotics contributes to regulating the balance of intestinal microbiota.
为研究益生菌和合生元对小鼠急性结肠炎炎症及微生物群的影响。将C57BL/6J小鼠随机分为4组。每组10只小鼠，除空白对照组外，其余各组给予2.5%葡聚糖硫酸钠（DSS）饮用水5天。除模型对照组外，另外两组分别给予益生菌和合生元。益生菌由……组成，而合生元由上述益生菌、菊粉和低聚半乳糖组成。收集不同时期的粪便和黏膜样本，通过16s rDNA测序分析肠道菌群的差异。（1）益生菌组和合生元组的病理评分分别为5.40±2.79和7.25±2.87，显著低于模型对照组的27.00±7.94。模型对照组、益生菌组和合生元组与空白对照组相比，菌群多样性降低，……增加，……减少。每组黏膜微生物群在丰度和种类上与粪便微生物群不同，……在黏膜中更常见。益生菌和合生元可减轻小鼠急性结肠炎的炎症。有益菌属与有害菌属失衡是急性结肠炎的特征。补充益生菌和合生元有助于调节肠道微生物群的平衡。
