沙门氏菌不耐热肠毒素的免疫原性和抗原性特征

Immunogenic and antigenic characteristics of Salmonella heat-labile enterotoxin.

作者信息

Kaura Y K, Sharma V K

机构信息

Department of Veterinary Microbiology, Haryana Agricultural University, Hisar, India.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1988 May;268(3):386-404. doi: 10.1016/s0176-6724(88)80024-5.

DOI:10.1016/s0176-6724(88)80024-5

PMID:3136609

Abstract

The intramuscular immunization of rabbits with enterotoxin of S. weltevreden failed to provide protection against challenges with homologous Salmonella enterotoxin as well as heterologous enterotoxins (cholera toxin or E. coli LT). Similar results were obtained in rabbits immunized with cholera toxin, choleragenoid and E. coli LT. However, Salmonella antitoxin contained neutralizing antibodies against Salmonella enterotoxin (but not against cholera toxin) and thus was capable of neutralizing Salmonella enterotoxin when tested for skin permeability reaction. Immunodiffusion experiments showed that antitoxin prepared against the enterotoxin of one of the strains of S. weltevreden formed precipitin bands with enterotoxin preparations of 5 strains of S. weltevreden and 2 strains of S. anatum. However, Salmonella antitoxin failed to form precipitin bands with enterotoxins of other heterologous Salmonella species (S. dublin, S. enteritidis, S. hindmarsh and S. newport), cholera toxin and E. coli LT. The immunoelectrophoretic studies corroborated the results obtained by double immunodiffusion experiments. However, both Salmonella and cholera toxins migrated electrophoretically toward the cathode and resembled globulin in this respect. Salmonella enterotoxin, though immunogenic, yet proved unprotective through the parenteral route and appears to be antigenically distinct from cholera and E. coli enterotoxins.

摘要

用韦氏沙门氏菌肠毒素对家兔进行肌肉注射免疫，未能使其免受同源沙门氏菌肠毒素以及异源肠毒素（霍乱毒素或大肠杆菌不耐热肠毒素）攻击的影响。在用霍乱毒素、类霍乱原和大肠杆菌不耐热肠毒素免疫的家兔中也得到了类似结果。然而，沙门氏菌抗毒素含有针对沙门氏菌肠毒素的中和抗体（但不针对霍乱毒素），因此在进行皮肤通透性反应测试时能够中和沙门氏菌肠毒素。免疫扩散实验表明，用韦氏沙门氏菌一个菌株的肠毒素制备的抗毒素与5个韦氏沙门氏菌菌株和2个鸭沙门氏菌菌株的肠毒素制剂形成了沉淀带。然而，沙门氏菌抗毒素未能与其他异源沙门氏菌（都柏林沙门氏菌、肠炎沙门氏菌、欣德马什沙门氏菌和新港沙门氏菌）的肠毒素、霍乱毒素和大肠杆菌不耐热肠毒素形成沉淀带。免疫电泳研究证实了双向免疫扩散实验的结果。然而，沙门氏菌毒素和霍乱毒素在电泳时均向阴极迁移，在这方面与球蛋白相似。沙门氏菌肠毒素虽然具有免疫原性，但经非肠道途径证明无保护作用，且在抗原性上似乎与霍乱毒素和大肠杆菌肠毒素不同。