腰果苹果汁补充剂可通过减少高强度运动后氧化应激来提高白细胞计数，无论训练与否。

Cashew apple juice supplementation enhances leukocyte count by reducing oxidative stress after high-intensity exercise in trained and untrained men.

机构信息

Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand.

Exercise and Nutrition Sciences and Innovation Research Unit, Burapha University, Chonburi, 20131, Thailand.

出版信息

J Int Soc Sports Nutr. 2019 Jul 31;16(1):31. doi: 10.1186/s12970-019-0299-2.

DOI:10.1186/s12970-019-0299-2

PMID:31366352

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6670219/

Abstract

BACKGROUND

Cashew apple juice (CAJ) was shown to improve immunological mechanisms by regulating a balance between reactive oxygen species and antioxidant concentrations. However, no study exploring the effects of the CAJ and training status on the immune system and oxidative stress induced by exercise. Therefore, we investigated the effects of CAJ supplementation primarily on leukocyte counts and secondary on oxidative stress and cortisol changes after high-intensity exercise in trained and untrained men.

METHODS

Ten moderately (endurance) trained (Age = 21.5 ± 0.97 yr., VO2max = 45.6 ± 4.12 mL/kgBM/min) and ten sedentary men (Age = 20.4 ± 2.72 yr., VO2peak = 32.2 ± 7.26 mL/kgBM/min) were randomized to ingest either daily CAJ or a placebo at 3.5 mL/kgBM/day for 4 weeks, with a four-week washout period. Before and after each period, they performed 20-min, high-intensity cycling (85% VO2max), with blood samples collected immediately preceding and the following exercise. Samples were analyzed to determine leukocyte counts, malondialdehyde, 8-isoprostane, and cortisol concentrations. A repeated measures analysis of variance was used to examine the effects of supplement and training status over time with an alpha level of 0.05.

RESULTS

There was no interaction between supplement and training status on those variables before and after exercise. However, CAJ raised resting neutrophil counts and exercise-induced leukocyte counts in the trained group (all p < 0.05). Besides, CAJ significantly reduced plasma malondialdehyde concentrations at rest and after exercise and reduced the post-exercise plasma 8-isoprostane concentration in both groups of subjects (p < 0.05). Moreover, CAJ reduced plasma cortisol after exercise in the untrained subjects.

CONCLUSIONS

We suggest that 4-week CAJ supplementation can enhance exercise-induced leukocyte and resting neutrophil counts in trained men. The possible mechanism is a reduction in oxidative stress. However, the supplementation did not change the immune responses of untrained men, but it did reduce stress hormone concentrations.

TRIAL REGISTRATION NUMBER

TCTR20181127002 Registered 26 November 2018 "retrospectively registered".

摘要

背景

腰果苹果汁（CAJ）通过调节活性氧和抗氧化浓度之间的平衡来改善免疫机制。然而，尚无研究探讨 CAJ 和训练状态对运动诱导的免疫系统和氧化应激的影响。因此，我们主要研究了 CAJ 补充剂对高强度运动后白细胞计数的影响，并次要研究了对氧化应激和皮质醇变化的影响，该研究纳入了训练有素和未经训练的男性。

方法

10 名适度（耐力）训练的男性（年龄=21.5±0.97 岁，最大摄氧量=45.6±4.12 mL/kgBM/min）和 10 名久坐不动的男性（年龄=20.4±2.72 岁，最大摄氧量=32.2±7.26 mL/kgBM/min）被随机分为每天摄入 CAJ 或安慰剂 3.5mL/kgBM/天，持续 4 周，之后有 4 周的洗脱期。在每个周期前后，他们进行 20 分钟的高强度自行车运动（85%最大摄氧量），运动前和运动后立即采集血液样本。分析样本以确定白细胞计数、丙二醛、8-异前列腺素和皮质醇浓度。采用重复测量方差分析来检验补充剂和训练状态随时间的变化，显著性水平为 0.05。

结果

在运动前后，补充剂和训练状态之间没有相互作用。然而，CAJ 提高了训练组的静息中性粒细胞计数和运动诱导的白细胞计数（均 p<0.05）。此外，CAJ 显著降低了两组受试者的静息和运动后血浆丙二醛浓度，并降低了运动后两组受试者的血浆 8-异前列腺素浓度（均 p<0.05）。此外，CAJ 降低了未训练受试者运动后的血浆皮质醇。