Papanikolaou Konstantinos, Draganidis Dimitrios, Chatzinikolaou Athanasios, Laschou Vassiliki C, Georgakouli Kalliopi, Tsimeas Panagiotis, Batrakoulis Alexios, Deli Chariklia K, Jamurtas Athanasios Z, Fatouros Ioannis G
School of Physical Education, Sport Sciences and Dietetics, University of Thessaly, Karies, 42100, Trikala, Greece.
School of Physical Education and Sport Sciences, Democritus University of Thrace, 69100, Komotini, Greece.
Trials. 2019 Jul 31;20(1):469. doi: 10.1186/s13063-019-3557-3.
Muscle satellite cells (SCs) are crucial for muscle regeneration following muscle trauma. Acute skeletal muscle damage results in inflammation and the production of reactive oxygen species (ROS) which may be implicated in SCs activation. Protection of these cells from oxidative damage is essential to ensure sufficient muscle regeneration. The aim of this study is to determine whether SCs activity under conditions of aseptic skeletal muscle trauma induced by exercise is redox-dependent.
METHODS/DESIGN: Based on the SCs content in their vastus lateralis skeletal muscle, participants will be classified as either high or low respondents. In a randomized, double-blind, crossover, repeated-measures design, participants will then receive either placebo or N-acetylcysteine (alters redox potential in muscle) during a preliminary 7-day loading phase, and for eight consecutive days following a single bout of intense muscle-damaging exercise. In both trials, blood samples and muscle biopsies will be collected, and muscle performance and soreness will be measured at baseline, pre-exercise, 2 and 8 days post exercise. Biological samples will be analyzed for redox status and SCs activity. Between trials, a 4-week washout period will be implemented.
This study is designed to investigate the impact of redox status on SCs mobilization and thus skeletal muscle potential for regeneration under conditions of aseptic inflammation induced by exercise. Findings of this trial should provide insight into (1) molecular pathways involved in SCs recruitment and muscle healing under conditions of aseptic skeletal muscle trauma present in numerous catabolic conditions and (2) whether skeletal muscle's potential for regeneration depends on its basal SCs content.
ClinicalTrials.gov, ID: NCT03711838 . Registered on 19 Oct 2018.
肌肉卫星细胞（SCs）对于肌肉创伤后的肌肉再生至关重要。急性骨骼肌损伤会导致炎症反应和活性氧（ROS）的产生，这可能与SCs的激活有关。保护这些细胞免受氧化损伤对于确保足够的肌肉再生至关重要。本研究的目的是确定在运动诱导的无菌性骨骼肌创伤条件下SCs的活性是否依赖于氧化还原状态。
方法/设计：根据股外侧肌中SCs的含量，参与者将被分为高反应者或低反应者。在随机、双盲、交叉、重复测量设计中，参与者将在初步的7天负荷期内接受安慰剂或N-乙酰半胱氨酸（改变肌肉中的氧化还原电位），并在单次剧烈肌肉损伤运动后的连续八天内接受。在两项试验中，都将采集血样和肌肉活检样本，并在基线、运动前、运动后2天和8天测量肌肉性能和酸痛程度。将对生物样本进行氧化还原状态和SCs活性分析。在两项试验之间，将实施为期4周的洗脱期。
本研究旨在调查氧化还原状态对SCs动员的影响，从而研究在运动诱导的无菌性炎症条件下骨骼肌的再生潜力。该试验的结果应能深入了解（1）在许多分解代谢条件下存在的无菌性骨骼肌创伤情况下，参与SCs募集和肌肉愈合的分子途径，以及（2）骨骼肌的再生潜力是否取决于其基础SCs含量。
ClinicalTrials.gov，ID：NCT03711838。于2018年10月19日注册。
