依沙吖啶诱导甲状腺癌细胞凋亡和分化。

Ethacridine Induces Apoptosis and Differentiation in Thyroid Cancer Cells .

机构信息

Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C.

Department of Surgery, MacKay Memorial Hospital and Mackay Medical College, Taipei, Taiwan, R.O.C.

出版信息

Anticancer Res. 2019 Aug;39(8):4095-4100. doi: 10.21873/anticanres.13567.

DOI:10.21873/anticanres.13567

PMID:31366493

Abstract

BACKGROUND/AIM: Ethacridine is used as a topical antiseptic as well as for second-trimester abortion. Recent studies showed that ethacridine is an inhibitor of poly(ADP-ribose) glycohydrolase (PARG) and an activator of the transcriptional coactivator with PDZ-binding motif (TAZ). This study examined the effects of ethacridine on thyroid cancer cells.

MATERIALS AND METHODS

Thyroid cancer cell lines (FTC133 and SW1736) and thyroid follicular epithelial cells (Nthy-ori 3-1) were treated with ethacridine. Viability, clonogenicity, cell-cycle distribution, and apoptosis were evaluated. The expression of thyroid differentiation markers (TTF-1, PAX8, and NIS) was determined by real-time PCR.

RESULTS

Ethacridine suppressed cell growth and clonogenic ability of thyroid cancer cells in a time- and dose-dependent manner (p<0.001). No cell-cycle arrest was found, but ethacridine dose-dependently induced apoptosis of thyroid cancer cells (p<0.001). The PAX8 and NIS expressions were significantly increased in SW1736 (3.41-fold and 1.53-fold, respectively) and Nthy-ori 3-1 cells (2.73-fold and 4.12-fold, respectively).

CONCLUSION

Ethacridine elicits apoptotic cell death in thyroid cancer cells and promotes differentiation in a subset of thyroid follicular cells.

摘要

背景/目的：依沙吖啶可用作局部防腐剂和中期妊娠流产药物。最近的研究表明，依沙吖啶是聚 ADP-核糖水解酶（PARG）的抑制剂和 PDZ 结合基序转录共激活剂（TAZ）的激活剂。本研究探讨了依沙吖啶对甲状腺癌细胞的影响。

材料和方法

用依沙吖啶处理甲状腺癌细胞系（FTC133 和 SW1736）和甲状腺滤泡上皮细胞（Nthy-ori 3-1）。评估细胞活力、集落形成能力、细胞周期分布和细胞凋亡。通过实时 PCR 测定甲状腺分化标志物（TTF-1、PAX8 和 NIS）的表达。

结果

依沙吖啶以时间和剂量依赖的方式抑制甲状腺癌细胞的生长和集落形成能力（p<0.001）。未发现细胞周期停滞，但依沙吖啶剂量依赖性诱导甲状腺癌细胞凋亡（p<0.001）。PAX8 和 NIS 在 SW1736（分别增加 3.41 倍和 1.53 倍）和 Nthy-ori 3-1 细胞（分别增加 2.73 倍和 4.12 倍）中的表达显著增加。