Chen Jiali, Tan Mengxia, Zou Lisi, Liu Xunhong, Chen Shuyu, Shi Jingjing, Chen Cuihua, Wang Chengcheng, Mei Yuqi
College of Pharmacy, Nanjing University of Chinese Medicine.
Chem Pharm Bull (Tokyo). 2019;67(8):839-848. doi: 10.1248/cpb.c19-00255.
Panacis Japonici Rhizoma (PJR) contains various kinds of saponins, which possesses extensive pharmacological activities, but studies of comprehensive analysis of its saponins were limited. Thus, ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS) and ultra-fast liquid chromatography coupled with triple quadrupole-linear ion trap tandem mass spectrometry (UFLC-QTRAP-MS/MS) methods were established for the qualitative and quantitative analysis of the saponins in PJR, separately. Fifty three saponins in PJR were identified by UFLC-Triple TOF-MS/MS method, 23 saponins of which were unequivocally identified by reference substances. In addition, fragmentation pathways of different types of saponins were preliminarily deduced by fragmentation behavior of 53 saponins. Furthermore, the simultaneous determination of the contents of 13 saponins in PJR samples harvested at different times were analyzed by UFLC-QTRAP-MS/MS method. Furthermore, the quality of the samples was evaluated by grey relational analysis. This study might be beneficial to the quality assessment and control of PJR. Meanwhile, it might provide the basic information for confirming its optimal harvested period.
竹节参含有多种皂苷，具有广泛的药理活性，但对其皂苷的综合分析研究有限。因此，分别建立了超快速液相色谱-三重四极杆-飞行时间串联质谱法（UFLC-Triple TOF-MS/MS）和超快速液相色谱-三重四极杆-线性离子阱串联质谱法（UFLC-QTRAP-MS/MS）用于竹节参中皂苷的定性和定量分析。采用UFLC-Triple TOF-MS/MS法鉴定出竹节参中的53种皂苷，其中23种皂苷通过对照品得到明确鉴定。此外，根据53种皂苷的裂解行为初步推导了不同类型皂苷的裂解途径。进一步采用UFLC-QTRAP-MS/MS法同时测定不同采收期竹节参样品中13种皂苷的含量。此外，通过灰色关联分析对样品质量进行评价。本研究可能有助于竹节参的质量评价和控制。同时，可为确定其最佳采收期提供基础资料。
