Wathoni Nasrul, Rusdiana Taofik, Hasanah Aliya Nur, Muhtadi Ahmad, Pratiwi Elasari Dwi, Mahmudah Ripa'tul, Mohammed Ahmed Fouad Abdelwahab, Okajima Maiko, Kaneko Tatsuo, Arima Hidetoshi
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University Padjadjaran.
Graduate School of Pharmaceutical Sciences, Kumamoto University.
Chem Pharm Bull (Tokyo). 2019;67(8):849-854. doi: 10.1248/cpb.c19-00291.
Regenerative therapy with keratinocyte growth factor (KGF) is a novel therapeutic approach for treatment of chronic wounds. However, KGF cannot be used directly to the wound site due to its physicochemical instability. In previous study, sacran, a natural megamolecular polysaccharide, showed potential properties as a biomaterial for hydrogel film in wound healing. In this study, we fabricated sacran hydrogel film containing KGF (Sac/KGF-HF) and evaluated the effects of Sac/KGF-HF on fibroblasts migration and re-epithelialization process. We successfully prepared a homogenous and -amorphous Sac/KGF-HF by a casting method. In addition, Sac/KGF-HF had a high swelling ratio and flexibility. Sac/KGF-HF promoted a migration process of NIH3T3 cells and improved wound healing ability in mice with a percentage of wound closure reaching 90.4% at 9 d. Interestingly, the addition of KGF in Sac-HF considerably increased the number of epithelial cells compared to control, which is important in the re-epithelialization process. It could be concluded that KGF in Sac-HF has the potential for promoting Sac-HF abilities in wound healing process.
角质形成细胞生长因子（KGF）再生疗法是治疗慢性伤口的一种新型治疗方法。然而，由于其物理化学不稳定性，KGF不能直接用于伤口部位。在先前的研究中，天然大分子多糖硫酸软骨素显示出作为伤口愈合水凝胶膜生物材料的潜在特性。在本研究中，我们制备了含有KGF的硫酸软骨素水凝胶膜（Sac/KGF-HF），并评估了Sac/KGF-HF对成纤维细胞迁移和再上皮化过程的影响。我们通过流延法成功制备了均匀且无定形的Sac/KGF-HF。此外，Sac/KGF-HF具有高溶胀率和柔韧性。Sac/KGF-HF促进了NIH3T3细胞的迁移过程，并提高了小鼠的伤口愈合能力，在第9天时伤口闭合率达到90.4%。有趣的是，与对照组相比，在Sac-HF中添加KGF显著增加了上皮细胞的数量，这在再上皮化过程中很重要。可以得出结论，Sac-HF中的KGF在伤口愈合过程中具有促进Sac-HF功能的潜力。
