Hosomi Ryota, Fukunaga Kenji, Nagao Toshihiro, Shiba Shunichi, Miyauchi Kazumasa, Yoshida Munehiro, Takahashi Koretaro
Faculty of Chemistry, Materials, and Bioengineering, Kansai University.
Osaka Research Institute of Industrial Science and Technology, Morinomiya Center.
J Oleo Sci. 2019;68(8):781-792. doi: 10.5650/jos.ess19103.
The possibility of improving brain function coupled with its preferential uptake in the brain has garnered attention for docosahexaenoic acid-bound lysophosphatidylcholine (DHA-LPC). However, studies focusing on the health benefits of dietary DHA-LPC are lacking. We prepared a dietary oil rich in DHA-LPC (DHA-LPC rich oil) via enzymatic modification of phospholipids (PL) extracted from squid (Todarodes pacificus) meal and purification of active carbon, ion exchange resin, and silica gel. We then examined the effects of dietary DHA-LPC rich oil on male Wistar rats by evaluating serum and liver lipid profiles, fatty acid (FA) metabolizing enzyme activity, and the FA composition of serum and brain. The rats were fed a basal diet containing either soybean oil alone (7%) or soybean oil (4.5%) with DHA-LPC rich oil (2.5%) for 28 days, and then evaluated. The rats fed the diet containing DHA-LPC rich oil showed reduced triacylglycerol concentration due, in part, to the enhancement of carnitine palmitoyltransferase 2 and acyl-CoA oxidase activities and suppression of acetyl-CoA carboxylase and glucose-6-phosphate dehydrogenase activities in the liver. Moreover, the dietary DHA-LPC rich oil moderately increased DHA in the FA composition of the rat hippocampus, which may be due to elevated DHA composition in serum LPC. These results suggest that DHA-LPC rich oil has hypolipidemic effect and moderate increase in hippocampal DHA amount in normal rats.
二十二碳六烯酸结合溶血磷脂酰胆碱（DHA-LPC）因具有改善脑功能的可能性及其在大脑中的优先摄取而受到关注。然而，针对膳食中DHA-LPC健康益处的研究却很匮乏。我们通过对从太平洋褶柔鱼粉中提取的磷脂（PL）进行酶促修饰，并经活性炭、离子交换树脂和硅胶纯化，制备了富含DHA-LPC的食用油（DHA-LPC富集油）。然后，我们通过评估血清和肝脏脂质谱、脂肪酸（FA）代谢酶活性以及血清和大脑的FA组成，研究了膳食中DHA-LPC富集油对雄性Wistar大鼠的影响。将大鼠分别喂食仅含大豆油（7%）的基础日粮或含大豆油（4.5%）与DHA-LPC富集油（2.5%）的基础日粮28天，然后进行评估。喂食含DHA-LPC富集油日粮的大鼠甘油三酯浓度降低，部分原因是肝脏中肉碱棕榈酰转移酶2和酰基辅酶A氧化酶活性增强，以及乙酰辅酶A羧化酶和葡萄糖-6-磷酸脱氢酶活性受到抑制。此外，膳食中DHA-LPC富集油适度增加了大鼠海马体FA组成中的DHA含量，这可能是由于血清LPC中DHA组成升高所致。这些结果表明，DHA-LPC富集油对正常大鼠具有降血脂作用，并能适度增加海马体中的DHA含量。
