Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka.
Tea Science Center, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka.
Biol Pharm Bull. 2019;42(8):1402-1408. doi: 10.1248/bpb.b19-00325.
Beta-cryptoxanthin (β-CRX, (3R)-β, β-caroten-3-ol) is an oxygenated carotenoid and a potent antioxidant that is abundant in Satsuma mandarin orange (Citrus unshiu MARC.), which is the most popular fruit in Japan. Since our preliminary data suggested that the ingestion of β-CRX had an anti-stress effect in female participants, the effect was evaluated in another set of female participants. The study design was a double-blind group comparison and participants (n = 23) were randomly assigned to β-CRX-rich orange juice or placebo (β-CRX was removed from orange juice) groups. β-CRX or placebo juice (125 mL, after breakfast) were consumed from 1 week prior to pharmacy practice and continued for 5 d into the practice period. Salivary α-amylase activity (sAA), a marker of sympathetic nervous system activity, was significantly higher in the evening than in the morning in the placebo-group during pharmacy practice, but not in the β-CRX-group. This result supports the anti-stress effect of β-CRX. The dose-dependency of β-CRX was observed in male mice that were loaded with stress. These results indicate that the ingestion of β-CRX is helpful to reduce stress.
β-隐黄质（β-CRX，（3R）-β，β-胡萝卜素-3-醇）是一种含氧类胡萝卜素，具有很强的抗氧化作用，在日本最受欢迎的水果温州蜜柑（Citrus unshiu MARC.）中含量丰富。由于我们的初步数据表明，β-CRX 的摄入对女性参与者有抗应激作用，因此在另一组女性参与者中评估了该作用。该研究设计为双盲分组比较，参与者（n=23）随机分配到富含β-CRX 的橙汁或安慰剂（橙汁中去除了β-CRX）组。β-CRX 或安慰剂橙汁（125 毫升，早餐后）在药房实习前一周开始服用，并在实习期间继续服用 5 天。在药房实习期间，安慰剂组的唾液淀粉酶活性（sAA），交感神经系统活动的标志物，晚上明显高于早上，但在β-CRX 组则不然。这一结果支持了β-CRX 的抗应激作用。在应激负荷的雄性小鼠中观察到了β-CRX 的剂量依赖性。这些结果表明，摄入β-CRX 有助于减轻压力。
