Department of Phytochemistry and Plant Systematics, Division of Pharmaceutical Industries, National Research Center, Dokki, Cairo, Egypt.
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.
Sci Rep. 2019 Jul 31;9(1):11122. doi: 10.1038/s41598-019-46946-7.
Reactive oxygen species (ROS) are involved in the pathophysiology of several health disorders, among others inflammation. Polyphenols may modulate ROS related disorders. In this work, thirty-two phenolic compounds were tentatively identified in a leaf extract from Eugenia supra-axillaris Spring. ex Mart. using HPLC-MS/MS, five of which were also individually isolated and identified. The extract displayed a substantial in vitro antioxidant potential and was capable of decreasing ROS production and hsp-16.2 expression under oxidative stress conditions in vivo in the Caenorhabditis elegans model. Also, the extract showed higher inhibitory selectivity towards COX-2 than COX-1 in vitro with higher selectivity towards COX-2 than that of diclofenac. The extract also exhibited anti-inflammatory properties: It attenuated the edema thickness in a dose dependent fashion in carrageenan-induced hind-paw odema in rats. In addition, the extract reduced the carrageenan-induced leukocyte migration into the peritoneal cavity at the highest dose. Furthermore, the extract showed antipyretic and analgesic activities in a mouse model. Eugenia supra-axillaris appears to be a promising candidate in treating inflammation, pain and related oxidative stress diseases.
活性氧（ROS）参与了多种健康障碍的病理生理学，包括炎症。多酚可能调节与 ROS 相关的疾病。在这项工作中，使用 HPLC-MS/MS 从 Eugenia supra-axillaris Spring。ex Mart. 的叶提取物中暂定鉴定了 32 种酚类化合物，其中 5 种也被单独分离和鉴定。该提取物在体外具有显著的抗氧化潜力，并且能够在体内减少 Caenorhabditis elegans 模型中氧化应激条件下的 ROS 产生和 hsp-16.2 表达。此外，该提取物在体外对 COX-2 的抑制选择性高于 COX-1，对 COX-2 的选择性高于双氯芬酸。该提取物还表现出抗炎特性：它以剂量依赖性方式减轻角叉菜胶诱导的大鼠后爪水肿的肿胀厚度。此外，该提取物在最高剂量下减少了角叉菜胶诱导的白细胞向腹腔内迁移。此外，该提取物在小鼠模型中表现出解热和镇痛作用。Eugenia supra-axillaris 似乎是治疗炎症、疼痛和相关氧化应激疾病的有前途的候选药物。
