Kim Ho Myeong, Choi In Seong, Lee Seoyoun, Hwang In Min, Chun Ho Hyun, Wi Seung Gon, Kim Jin-Cheol, Shin Tae Young, Kim Jong Cheol, Kim Jae Su, Kim Junheon, Park Hae Woong
R&D Division, World Institute of Kimchi, 86 Kimchi-ro, Nam-gu, Gwangju, 61755 Republic of Korea.
2Asian Pear Research Institute, Chonnam National University, Gwangju, 61186 Republic of Korea.
Biotechnol Biofuels. 2019 Jul 25;12:188. doi: 10.1186/s13068-019-1530-8. eCollection 2019.
Biorefineries are widely recognized as the most feasible solution to the problem of achieving environmental sustainability along with economic growth. Furthermore, pine wilt disease has caused severe environmental and economic damage worldwide to date. Herein, a highly efficient, advanced process for producing destruxins (DTXs) from (MCT) is reported, along with an application strategy.
The acetic acid-sodium chlorite pretreatment of MCT (AASC-MCT) is found to improve the monosaccharide production. Through biocatalytic conversion processes (simultaneous saccharification and cultivation), JEF-279 can efficiently produce DTXs from 1% (w/v) AASC-MCT, i.e., DTX E (334.8 mg/L), A (288.8 mg/L), and B (48.6 mg/L). (MA, Japanese pine sawyer) is known to act as a mediator transferring to pinewood. As is associated with the occurrence of pine wilt disease, biological control of MA is a major strategy or controlling this disease. In this study, upon the application of a mixture of DTXs and protease-containing culture filtrate (PCF), complete mortality of MA is observed after a 5-day incubation. The MA immune system response is believed to cause an overexpression of actin and tropomyosin as a defense mechanism against the flaccid paralysis induced by the DTXs and PCF treatment.
These results suggest that MCT can be used as a major feedstock in the biorefinery industry and that DTXs can be applied as an insecticide for biological control of pine wilt disease via MA termination.
生物精炼厂被广泛认为是实现环境可持续性与经济增长问题的最可行解决方案。此外，迄今为止，松材线虫病已在全球范围内造成了严重的环境和经济破坏。在此，报道了一种从[具体物质未给出，推测为MCT（松材线虫病死木）]生产 destruxins（DTXs）的高效先进工艺以及应用策略。
发现MCT的醋酸 - 亚氯酸钠预处理（AASC - MCT）可提高单糖产量。通过生物催化转化过程（同步糖化和培养），JEF - 279能够从1%（w/v）的AASC - MCT中高效生产DTXs，即DTX E（334.8 mg/L）、A（288.8 mg/L）和B（48.6 mg/L）。[具体物质未给出，推测为MA（日本松材线虫）]已知可作为将[具体物质未给出]转移到松木的介质。由于[具体物质未给出]与松材线虫病的发生有关，对MA的生物防治是控制这种疾病的主要策略。在本研究中，应用DTXs和含蛋白酶的培养滤液（PCF）的混合物后，经5天培养观察到MA全部死亡。据信MA免疫系统反应会导致肌动蛋白和原肌球蛋白过度表达，作为对抗DTXs和PCF处理诱导的弛缓性麻痹的防御机制。
这些结果表明，MCT可作为生物精炼行业的主要原料，并且DTXs可作为杀虫剂通过杀死MA用于松材线虫病的生物防治。
