Lin Tachen, Chen Chunyeh, Chen Bangyuan, Shaw Jeifu, Chen Yuhshuen
College of Food Engineering Beibu Gulf University Qinzhou China.
Guangxi Colleges and University Key Laboratory of Development and High-value Utilization of Beibu Gulf Seafood Resources Beibu Gulf University Qinzhou China.
Food Sci Nutr. 2019 Jun 3;7(7):2336-2344. doi: 10.1002/fsn3.1079. eCollection 2019 Jul.
It is important that exopolysaccharides (EPS) of lactic acid bacteria (LAB) with antioxidant activities are produced economically, as it can confer beneficial effects on human health. A model of production possibility curve (PPC) was conducted to the optimal productivity of EPS for the purpose of economic production. The results revealed that the optimal productivity of EPS was approached to the set by NB (90%) and MRS (55%) broth from PPC with equation of PPC(Y) = 100-0.0335EXP(0.08X). The EPS productivity and yield of strain LaP with optimal production set (OPS) were, respectively, 291.0 ± 2.6 mg and 13.5 ± 0.7%, and the cost of OPS can be saved by about 31.6%, while that for strain BaP were 280.7 ± 2.5 mg and 13.0 ± 0.7%, respectively, and with 31.6% saved as well. Besides, the EPS produced from PPC mode has appropriate antioxidant ability with 34.6 ± 0.7% (LaP) and 37.6 ± 0.9 (%) (BaP) of DPPH radical scavenging activity under the economic cost. The strategy of controlling the medium composition not only could improve the productivity of EPS, but also enhance the antioxidant effects of EPS. Both LaP and BaP with antioxidant potential may be useful as supplements in the health-promoting food industry.
具有抗氧化活性的乳酸菌胞外多糖（EPS）能对人体健康产生有益影响，因此经济地生产这些胞外多糖很重要。为了实现经济生产，构建了生产可能性曲线（PPC）模型来确定EPS的最佳生产率。结果表明，根据PPC方程PPC(Y) = 100 - 0.0335EXP(0.08X)，NB（90%）和MRS（55%）肉汤接近EPS的最佳生产率。具有最佳生产设置（OPS）的LaP菌株的EPS生产率和产量分别为291.0 ± 2.6毫克和13.5 ± 0.7%，OPS成本可节省约31.6%；而BaP菌株的分别为280.7 ± 2.5毫克和13.0 ± 0.7%，成本也节省31.6%。此外，在经济成本下，PPC模式生产的EPS具有适当的抗氧化能力，LaP的DPPH自由基清除活性为34.6 ± 0.7%，BaP为37.6 ± 0.9%。控制培养基成分的策略不仅可以提高EPS的生产率，还能增强EPS的抗氧化效果。具有抗氧化潜力的LaP和BaP菌株都可能作为健康促进食品工业的补充剂发挥作用。
