Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
Clermont Université, Université Blaise Pascal, Institut Pascal UMR CNRS 6602, axe GePEB, 2 Avenue Blaise Pascal, TSA 60206, CS 60026, 63178 Aubière CEDEX, France.
Bioresour Technol. 2016 Aug;213:231-238. doi: 10.1016/j.biortech.2016.02.075. Epub 2016 Feb 26.
With the aim to optimize the production of exopolysaccharide (EPS) by Porphyridium marinum, cultures in photobioreactors were conducted on a modified Provasoli medium (P) and compared to a new medium (Pm) with an elemental composition of N0.0205S0.0597P0.005. Cultivation on this medium allowed the increase of EPS concentration up to 2.5gL(-1), without modification of the EPS productivity (0.096gL(-1)) and EPS structure. In a second time, photosynthetic activity of the strain was monitored as a function of irradiance and temperature, allowing improvement of kinetic parameters of growth and EPS production. A semi-continuous culture, carried out with the Pm medium, an optimal irradiance and temperature of respectively 360μmolphotonsm(-2)s(-1) and 28°C led to an EPS process productivity of 0.031gh(-1) instead of 0.020gh(-1) in batch culture.
为了优化盐生杜氏藻（Porphyridium marinum）胞外多糖（EPS）的生产，在光生物反应器中对改良的 Provasoli 培养基（P）和新的培养基（Pm）进行了培养，新培养基的元素组成分别为 N0.0205S0.0597P0.005。在该培养基上培养可将 EPS 浓度提高至 2.5gL(-1)，而不改变 EPS 生产力（0.096gL(-1)）和 EPS 结构。其次，监测了菌株的光合活性作为辐照度和温度的函数，从而改善了生长和 EPS 生产的动力学参数。在 Pm 培养基、最佳辐照度和温度分别为 360μmolphotonsm(-2)s(-1)和 28°C 的半连续培养中，EPS 过程生产力为 0.031gh(-1)，而分批培养中仅为 0.020gh(-1)。
