Cai Rentian, Wang Qiongqiong, Zhu Gongmin, Zhu Liying, Tao Zhen
Department of Infectious Diseases, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
Nuclear Medicine Department, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
PeerJ. 2019 Jul 22;7:e7321. doi: 10.7717/peerj.7321. eCollection 2019.
Key factors of pyroptosis play an important role in the inflammatory response to connective tissue disease (CTD). However, information on active and stable stages of CTD is scarce. To distinguish the differences of concentrations of C-reactive protein (CRP), caspase 1, caspase 4, caspase 5 and sCD14 in plasma between the patients with active and stable stages of CTD. A cohort study was conducted to recruit patients diagnosed with CTD of active phase and stable phase as well as health control. These data included the analysis of the concentration of sCD14, caspase 1, caspase 4 and caspase 5 in peripheral plasma by ELISA. The Wilcoxon rank-sum test was used to compare the two groups. The sex ratio and ages of the three groups were not different statistically. The concentrations of sCD14, caspase4 and caspase5 of plasma in the CTD of active phase and the stable phase as well as the health control. The concentration of caspase 1 in active phase of CTD (470.19 [422.33-513.14] pmol/L) was significantly higher than that in stable group (203.95 [160.94-236.12] pmol/L) and healthy control (201.65 [191.11-240.35] pmol/L] pmol/L) ( < 0.001, both), but there was no significant difference between stable group and healthy control ( = 0.2312). Similarly, the concentration of CRP in the active phase of CTD (8.96 [3.06-20.28] mg/L) was significantly higher than that in the stable group (3.00 [1.30-11.40] mg/L) and the healthy control (3.70 [2.30-4.73] mg/L) ( = 0.0013, = 0.0006, respectively), but there was no significant difference between the stable group and the healthy control ( = 0.3205). However, there were no significant differences in the concentration of sCD14, caspase 4 and caspase 5 in the active phase of CTD and the stable group as well as the health group. Consequently, the patients of the active phase of CTD showed increased expression of caspase 1.
细胞焦亡的关键因子在结缔组织病（CTD）的炎症反应中起重要作用。然而，关于CTD活动期和稳定期的信息却很匮乏。为了区分CTD活动期和稳定期患者血浆中C反应蛋白（CRP）、半胱天冬酶1、半胱天冬酶4、半胱天冬酶5和可溶性CD14（sCD14）浓度的差异。进行了一项队列研究，招募了诊断为CTD活动期和稳定期的患者以及健康对照。这些数据包括通过酶联免疫吸附测定（ELISA）分析外周血中sCD14、半胱天冬酶1、半胱天冬酶4和半胱天冬酶5的浓度。采用Wilcoxon秩和检验对两组进行比较。三组的性别比例和年龄在统计学上无差异。CTD活动期和稳定期以及健康对照血浆中sCD14、半胱天冬酶4和半胱天冬酶5的浓度情况。CTD活动期半胱天冬酶1的浓度（470.19[422.33 - 513.14]pmol/L）显著高于稳定组（203.95[160.94 - 236.12]pmol/L）和健康对照组（201.65[191.11 - 240.35]pmol/L）（均P<0.001），但稳定组与健康对照组之间无显著差异（P = 0.2312）。同样，CTD活动期CRP的浓度（8.96[3.06 - 20.28]mg/L）显著高于稳定组（3.00[1.30 - 11.40]mg/L）和健康对照组（3.70[2.30 - 4.73]mg/L）（分别为P = 0.0013，P = 0.0006），但稳定组与健康对照组之间无显著差异（P = 0.3205）。然而，CTD活动期与稳定组以及健康组之间sCD14、半胱天冬酶4和半胱天冬酶5的浓度无显著差异。因此，CTD活动期患者半胱天冬酶1的表达增加。
