Puch-Hau Carlos, Sánchez-Tapia Itzel A, Patiño-Suárez Victoria, Olvera-Novoa Miguel A, Gullian Klanian Mariel, Quintanilla-Mena Mercedes, Zapata-Pérez Omar
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV), Departamento de Recursos del Mar, Unidad Mérida, Km. 6 Antigua Carretera a Progreso, Apdo. Postal 73-Cordemex, 97310, Mérida, Yucatán, Mexico.
Universidad Marista de Mérida, Periférico Norte Tablaje Catastral 13941, Carretera Mérida-Progreso, P.O. Box 97300, Mérida, Yucatán, Mexico.
MethodsX. 2019 Jul 12;6:1627-1634. doi: 10.1016/j.mex.2019.07.010. eCollection 2019.
is a sea cucumber species of great ecological and economic relevance for Mexico and Central American and Caribbean countries; however, the protocols for the extraction of the nucleic acids have not yet been published. In this study, we describe the first protocols to obtain DNA and RNA from different tissues of which include the respiratory tree, gonad, longitudinal muscle bands, anterior intestine and cloaca. The extraction of high-quality DNA was performed using the DNeasy Blood & Tissue kit (Qiagen, Valencia, CA, USA) with minor modifications in different points of the protocol. Concerning the RNA, the method of TRIzol was used. This method is particularly advantageous in situations where cells or tissues are enriched for endogenous RNases or when the separation of cytoplasmic RNA from nuclear RNA is impractical. The methodologies used in this study allowed us to obtain DNA and RNA of high quality and integrity in the different tissues of which will be the basis for future genomic and transcriptomic studies. •The successful extraction of DNA and RNA was achieved in the different tissues of .•The concentrations of DNA and RNA obtained were adequate for a diversity of analyses at a molecular level.
是一种对墨西哥、中美洲和加勒比国家具有重大生态和经济意义的海参物种；然而，核酸提取方案尚未公布。在本研究中，我们描述了从其不同组织（包括呼吸树、性腺、纵肌带、前肠和泄殖腔）中获取DNA和RNA的首个方案。使用DNeasy Blood & Tissue试剂盒（Qiagen，美国加利福尼亚州瓦伦西亚）进行高质量DNA的提取，并在方案的不同点进行了微小修改。关于RNA，使用了TRIzol方法。该方法在细胞或组织富含内源性核糖核酸酶的情况下，或者从核RNA中分离细胞质RNA不切实际的情况下特别有利。本研究中使用的方法使我们能够在的不同组织中获得高质量和完整性的DNA和RNA，这将成为未来基因组和转录组研究的基础。•在的不同组织中成功提取了DNA和RNA。•获得的DNA和RNA浓度足以进行多种分子水平分析。
