Zhang Qinghua, Ji Ce, Ren Jianfeng, Zhang Qiuyue, Dong Xuehong, Zu Yao, Jia Liang, Li Weiming
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, China.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, China.
J Fish Dis. 2018 Jul;41(7):1049-1062. doi: 10.1111/jfd.12796. Epub 2018 Mar 23.
Zebrafish embryo and larva represent a useful in vivo model for identification of host innate immune responses to bacterial infection. Vibrio parahaemolyticus is a typical zoonotic pathogen worldwide that causes acute gastroenteritis in humans and vibriosis in fishes. However, the mechanism of the innate immune response in the zebrafish larvae infected by V. parahaemolyticus has not been clear. We analysed the transcriptomic profile of 3 days post-fertilization (dpf) zebrafish larvae immersed in V. parahaemolyticus 13 (Vp13) strain suspension for 2 hr. A total of 602 differentially expressed genes (DEGs) were identified in the infection group, of which 175 (29.07%) genes were upregulated and 427 (70.93%) genes were downregulated. These altered genes encoded complement and coagulation cascades, chemokine, TNF signalling pathway, NF-κB signalling pathway and JAK-STAT signalling pathway. Some significant DEGs, such as mmp13, cxcr4a, ccl20, hsp70, gngt, serpina1l, il8, cofilin and il11, were subjected to quantitative gene expression analysis, and the results were consistent with those of the transcriptome profile. These results clearly demonstrated that exposure to V. parahaemolyticus for 2 hr could activate innate immune response in 3dpf larvae by altered expression of downstream signalling pathway genes of pattern recognition receptors (PRRs). Our results also provide a useful reference for future analysis of signal transduction pathways and pathogenesis mechanisms underlying the systemic innate immune response to the external bacteria of V. parahaemolyticus.
斑马鱼胚胎和幼体是用于鉴定宿主对细菌感染的固有免疫反应的一种有用的体内模型。副溶血性弧菌是一种典型的全球人畜共患病原体，可导致人类急性肠胃炎和鱼类弧菌病。然而，副溶血性弧菌感染的斑马鱼幼体中固有免疫反应的机制尚不清楚。我们分析了受精后3天（dpf）的斑马鱼幼体在副溶血性弧菌13（Vp13）菌株悬液中浸泡2小时后的转录组概况。在感染组中总共鉴定出602个差异表达基因（DEG），其中175个（29.07%）基因上调，427个（70.93%）基因下调。这些改变的基因编码补体和凝血级联、趋化因子、TNF信号通路、NF-κB信号通路和JAK-STAT信号通路。对一些显著的DEG，如mmp13、cxcr4a、ccl20、hsp70、gngt、serpina1l、il8、cofilin和il11进行了基因表达定量分析，结果与转录组概况一致。这些结果清楚地表明，暴露于副溶血性弧菌2小时可通过模式识别受体（PRR）下游信号通路基因的表达改变激活3dpf幼体的固有免疫反应。我们的结果也为未来分析副溶血性弧菌对外界细菌的全身固有免疫反应的信号转导途径和发病机制提供了有用的参考。
