[对结肠造口母鸡进行的15N标记赖氨酸研究。2. 粪便中15N的排泄情况]
[Studies with 15N-labeled lysine in colostomized hens. 2. 15N excretion in feces].
作者信息
Gruhn K, Wiefel P
出版信息
Arch Tierernahr. 1983 May;33(4-5):349-62. doi: 10.1080/17450398309425690.
Over a period of four days colostomised hens were given 15N-lysine, and the development of 15N-excretion both in the TCA-soluble and the TCA-precipitable fraction of the faeces was measured over eight days. In both fractions the total, lysine, histidine and arginine N and 15N-excess (15N') was determined. The average apparent digestibility of 14N was 81.2% +/- 1.1% and of 15N' 93.2% +/- 0.7%. Labelled N is already excreted in faeces 3 hours after its application. The TCA-precipitable N is less strongly labelled than the TCA-soluble N. During the application of 15N' the labelling in faecal lysine is nearly one power of ten higher than in total N. The atom-% 15N' of the lysine could also be distinctly detected in arginine and histidine. The quotas of the total 15N' in faeces were 3.5% for arginine-15N' and 0.8% for histidine 15N'; 15N' can mainly be detected in the soluble fraction.
在四天的时间里，给行结肠造口术的母鸡喂食15N-赖氨酸，并在八天内测量粪便中三氯乙酸（TCA）可溶性部分和TCA沉淀部分中15N排泄的情况。在这两个部分中，测定了总氮、赖氨酸氮、组氨酸氮、精氨酸氮以及15N过量（15N'）。14N的平均表观消化率为81.2%±1.1%，15N'的平均表观消化率为93.2%±0.7%。标记的氮在施用后3小时就已在粪便中排泄。TCA沉淀氮的标记强度低于TCA可溶性氮。在施用15N'期间，粪便中赖氨酸的标记比总氮的标记高近一个数量级。赖氨酸的15N'原子百分比在精氨酸和组氨酸中也能明显检测到。粪便中精氨酸-15N'的总15N'配额为3.5%，组氨酸15N'的总15N'配额为0.8%；15N'主要在可溶性部分中检测到。
Zotero 插件