Dziedziech Alexis, Schmid Martin, Arefin Badrul, Kienzle Thomas, Krautz Robert, Theopold Ulrich
Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden.
Data Brief. 2019 Jul 9;25:104229. doi: 10.1016/j.dib.2019.104229. eCollection 2019 Aug.
Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization of two secretory proteins in , Prophenoloxidase (PPO2) and Transglutaminase-A (Tg) in hemocytes as well the clot with different tissue-specific drivers. Here we provide further data for the usefulness of the GFP-tagged version of the two crosslinking enzymes that are involved in clot hardening. The morphology of crystal cells is described using GFP-tagged PPO2 rather than with the use of antibodies in hemolymph preparations. The use of the GFP-tagged proteins PPO2 and Tg is shown in additional contexts.
经典和非经典分泌凝血因子的动力学 施密德等人，2019年。相关文章展示了两种分泌蛋白，即血细胞中的酚氧化酶原（PPO2）和转谷氨酰胺酶-A（Tg）以及具有不同组织特异性驱动因素的凝块的定位。在此，我们提供了更多数据，证明了参与凝块硬化的两种交联酶的绿色荧光蛋白（GFP）标记版本的实用性。使用GFP标记的PPO2而非血淋巴制剂中的抗体来描述晶体细胞的形态。GFP标记的蛋白PPO2和Tg在其他情况下也有应用。
