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使用绿色荧光蛋白标记的分泌蛋白(酚氧化酶原和转谷氨酰胺酶)对果蝇凝血和血细胞形态的数据。

Data on Drosophila clots and hemocyte morphologies using GFP-tagged secretory proteins: Prophenoloxidase and transglutaminase.

作者信息

Dziedziech Alexis, Schmid Martin, Arefin Badrul, Kienzle Thomas, Krautz Robert, Theopold Ulrich

机构信息

Stockholm University, Department of Molecular Biosciences, Svante Arrhenius Väg 20C, SE, 10691 Sweden.

出版信息

Data Brief. 2019 Jul 9;25:104229. doi: 10.1016/j.dib.2019.104229. eCollection 2019 Aug.

Abstract

Insect hemolymph coagulation: Kinetics of classically and non-classically secreted clotting factors Schmid et al., 2019. The linked article demonstrates the localization of two secretory proteins in , Prophenoloxidase (PPO2) and Transglutaminase-A (Tg) in hemocytes as well the clot with different tissue-specific drivers. Here we provide further data for the usefulness of the GFP-tagged version of the two crosslinking enzymes that are involved in clot hardening. The morphology of crystal cells is described using GFP-tagged PPO2 rather than with the use of antibodies in hemolymph preparations. The use of the GFP-tagged proteins PPO2 and Tg is shown in additional contexts.

摘要

昆虫血淋巴凝固

经典和非经典分泌凝血因子的动力学 施密德等人,2019年。相关文章展示了两种分泌蛋白,即血细胞中的酚氧化酶原(PPO2)和转谷氨酰胺酶-A(Tg)以及具有不同组织特异性驱动因素的凝块的定位。在此,我们提供了更多数据,证明了参与凝块硬化的两种交联酶的绿色荧光蛋白(GFP)标记版本的实用性。使用GFP标记的PPO2而非血淋巴制剂中的抗体来描述晶体细胞的形态。GFP标记的蛋白PPO2和Tg在其他情况下也有应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/89f6/6646922/d8f445eb644d/gr1.jpg

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