Willott E, Trenczek T, Thrower L W, Kanost M R
Department of Biochemistry, Kansas State University, Manhattan 66506-6100.
Eur J Cell Biol. 1994 Dec;65(2):417-23.
We have made 140 monoclonal antibodies to hemocytes (insect blood cells) from Manduca sexta. Four of these antibodies, when used in immunofluorescent microscopy of fixed hemocytes, distinguish the four main morphologically distinct hemocyte types. Plasmatocytes, granular cells, and oenocytoids are each recognized by a unique antibody specific to that type; spherulocytes are recognized by an antibody that also binds to plasmatocytes. When used in flow cytometry with nonfixed hemocytes, three of the four antibodies bind their respective cells; the oenocytoid marker failed to bind to any hemocytes. This set of four monoclonal antibodies may be useful for labeling individual cell types and for separating the different hemocyte types for further study of hemocyte functions.
我们已经制备了140种针对烟草天蛾血细胞（昆虫血细胞）的单克隆抗体。其中四种抗体，在用于固定血细胞的免疫荧光显微镜检查时，可区分出四种形态上明显不同的主要血细胞类型。浆细胞、颗粒细胞和绛色细胞分别由针对该类型的独特抗体识别；球形细胞由一种也能与浆细胞结合的抗体识别。当与未固定的血细胞一起用于流式细胞术时，四种抗体中的三种能结合各自对应的细胞；绛色细胞标记物未能与任何血细胞结合。这一组四种单克隆抗体可能有助于标记单个细胞类型，并分离不同的血细胞类型，以进一步研究血细胞功能。
