Kotnova A P, Lyanova B M, Dukhanina E A, Portseva T N, Ilyin Yu V, Georgieva S G, Stepchenko A G, Pankratova E V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia.
Dokl Biochem Biophys. 2019 May;486(1):181-183. doi: 10.1134/S1607672919030050. Epub 2019 Jul 31.
Thapsigargin (SERCA ATPase inhibitor) inhibited the S100A4 metastatic marker expression in MDA-MB231 breast cancer cells. We found that S100A4 gene transcription is regulated by Ca signaling pathways. We found that the synthesis of S100A4 mRNA and S100A4 protein in MDA-MB231 cells was effectively suppressed by thapsigargin at a concentration of 0.4-4 μM with retaining cell viability. We assume that the change in the gene transcription in response to disturbance of Ca homeostasis is directly involved in the remodeling of Ca signaling pathways.
毒胡萝卜素（SERCA ATP酶抑制剂）抑制了MDA - MB231乳腺癌细胞中S100A4转移标志物的表达。我们发现S100A4基因转录受钙信号通路调控。我们发现，在浓度为0.4 - 4μM的毒胡萝卜素作用下，MDA - MB231细胞中S100A4 mRNA和S100A4蛋白的合成被有效抑制，同时细胞活力得以保留。我们推测，响应钙稳态紊乱的基因转录变化直接参与了钙信号通路的重塑。
