Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, 31 To Yuen Street, Kowloon Tong, Hong Kong.
Cell Mol Life Sci. 2019 Dec;76(24):4861-4867. doi: 10.1007/s00018-019-03252-9. Epub 2019 Jul 31.
RNAs are responsible for mediating genetic information flow within the cell. RNA splicing, modification, trafficking, translation, and stability are all controlled at the transcript level. However, biological tools to study and manipulate them in a programmable fashion are currently limited. In this review, we summarize recent advances regarding available RNA-targeting systems discovered so far, including CRISPR-based technologies-Cas9 and Cas13, and programmable RNA-binding proteins-PUF and PPR. These tools allow transcript-specific manipulation in gene expression.
RNAs 负责在细胞内介导遗传信息流。RNA 的剪接、修饰、运输、翻译和稳定性都在转录水平上受到控制。然而，目前用于以可编程方式研究和操纵它们的生物学工具仍然有限。在这篇综述中，我们总结了迄今为止发现的可用 RNA 靶向系统的最新进展，包括基于 CRISPR 的技术-Cas9 和 Cas13，以及可编程 RNA 结合蛋白-PUF 和 PPR。这些工具允许对基因表达中的转录物进行特异性操作。
