Watanabe Takafumi, Hiramatsu Kohzy, Nishimura Kei, Ono Tamao
Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399-4598, Japan.
Laboratory of Animal Developmental Genetics, Faculty of Agriculture, Shinshu University, Minami-minowa 8304, Kami-ina, Nagano 399-4598, Japan.
J Poult Sci. 2018;55(3):199-203. doi: 10.2141/jpsa.0170185. Epub 2018 Jan 25.
Glucagon-like peptide (GLP)-1 is released from the intestinal L cells in response to food ingestion and stimulates insulin secretion from the pancreatic B cells, by binding to its specific receptor (GLP-1R), which is expressed on the pancreatic B cells in the mammalian pancreas. Previously, we demonstrated that chicken GLP-1R was expressed on the pancreatic D cells by using a specific antibody against chicken GLP-1R. In the present study, we compared the localization of GLP-1R in the pancreases of three avian species; white leghorn chicken, northern bobwhite, and common ostrich, using the double immunofluorescence technique. We found that the types of pancreatic islets in the northern bobwhite pancreas were similar to those found in the chicken pancreas. The ostrich pancreas contained several types of pancreatic islets. GLP-1R-immunoreactive cells were found in all types of pancreatic islets in both northern bobwhite and ostrich and expressed somatostatin immunoreactivity. The present results indicate that the pancreatic D cells are the target cells of GLP-1, and GLP-1 might play a physiological role via somatostatin in the avian species.
胰高血糖素样肽（GLP）-1在摄食后从肠道L细胞释放，通过与表达于哺乳动物胰腺胰岛B细胞上的特异性受体（GLP-1R）结合，刺激胰腺B细胞分泌胰岛素。此前，我们通过使用针对鸡GLP-1R的特异性抗体证明鸡GLP-1R在胰腺D细胞上表达。在本研究中，我们使用双重免疫荧光技术比较了三种鸟类（白来航鸡、北美鹑和普通鸵鸟）胰腺中GLP-1R的定位。我们发现北美鹑胰腺中的胰岛类型与鸡胰腺中的相似。鸵鸟胰腺包含几种类型的胰岛。在北美鹑和鸵鸟的所有类型胰岛中均发现了GLP-1R免疫反应性细胞，且这些细胞表达生长抑素免疫反应性。目前的结果表明胰腺D细胞是GLP-1的靶细胞，并且GLP-1可能通过生长抑素在鸟类中发挥生理作用。
