Lohmeyer J, Hadam M, Santoso S, Förster W, Schulz A, Pralle H
Department for Internal Medicine, University of Giessen, F.R.G.
Br J Haematol. 1988 Jul;69(3):335-43. doi: 10.1111/j.1365-2141.1988.tb02371.x.
A human myeloma cell line designated LOPRA-1 has been established from ascites fluid containing malignant plasma cells of a patient with IgA2/kappa multiple myeloma. The cultured cells which are Epstein-Barr virus (EBV) negative have retained the morphological, cytochemical, ultrastructural and immunophenotypical features of well-differentiated plasma cells. They express the plasma cell antigen PCA-1, the antigens CD28 (Kolt-2) and CD38 (OKT10), the transferrin-receptor (OKT9), and some epitopes of the CD24 antigen (HB8, VIB E3), but are negative for surface immunoglobulins. HLA class II antigens (HLA-DP, -DQ, -DR) and other B-cell markers such as CD10 (CALLA), CD19 (B4), CD20 (B1), CD21 (B2), CD22 (HD39), CD23 (MHM6), CD37 (BL14) and CD39 (G28-8) as analysed by both flow cytometry and immunocytochemistry (PAP/APAAP). With respect to immunoglobulin synthesis, two stable clones were selected by single cell cloning: clone LOPRA-1/5 synthesizes large amounts of alpha 2 heavy and kappa light chains, but secretes only small amounts of these molecules, whereas clone LOPRA-1/4 is clearly devoid of intracellular immunoglobulin heavy and light chains and thus appears to be a chain loss variant. Cytogenetic analysis revealed a pseudotriploid phenotype with several structurally abnormal marker chromosomes: 3n + -, 70, XX, -X, -1, -4, -6, -8, -8, -13, -16, +7, +18, +21, +i(1q), +i(1q), +6q-, +3mar.
一种名为LOPRA - 1的人骨髓瘤细胞系，是从一名IgA2/κ型多发性骨髓瘤患者含有恶性浆细胞的腹水中建立的。培养的细胞为Epstein - Barr病毒（EBV）阴性，保留了分化良好的浆细胞的形态、细胞化学、超微结构和免疫表型特征。它们表达浆细胞抗原PCA - 1、抗原CD28（Kolt - 2）和CD38（OKT10）、转铁蛋白受体（OKT9）以及CD24抗原的一些表位（HB8、VIB E3），但表面免疫球蛋白呈阴性。通过流式细胞术和免疫细胞化学（PAP/APAAP）分析，HLA - II类抗原（HLA - DP、- DQ、- DR）以及其他B细胞标志物如CD10（CALLA）、CD19（B4）、CD20（B1）、CD21（B2）、CD22（HD39）、CD23（MHM6）、CD37（BL14）和CD39（G28 - 8）均为阴性。关于免疫球蛋白合成，通过单细胞克隆选择了两个稳定的克隆：克隆LOPRA - 1/5合成大量α2重链和κ轻链，但仅分泌少量这些分子，而克隆LOPRA - 1/4明显缺乏细胞内免疫球蛋白重链和轻链，因此似乎是一个链缺失变体。细胞遗传学分析显示为假三倍体表型，有几条结构异常的标记染色体：3n + - ，70，XX，- X，- 1，- 4，- 6，- 8，- 8，- 13，- 16，+ 7，+ 18，+ 21，+ i(1q)，+ i(1q)，+ 6q - ，+ 3mar。
