Donelli A, Narni F, Tabilio A, Emilia G, Selleri L, Colo A, Zucchini P, Montagnani G, Torelli G, Torelli U
Int J Cancer. 1987 Sep 15;40(3):383-8. doi: 10.1002/ijc.2910400317.
We have established a new human plasma cell line from the peripheral blood of a patient with an IgA-kappa plasma-cell leukemia. Morphological, immunological, cytogenetic and molecular studies confirm that the cultured cells are derived from the same clone of leukemic plasma cell in vivo. The established cell line (MT3) grows in suspension, secretes high amounts of IgA kappa and exhibits morphological and ultrastructural characteristics of plasma cells. Surface marker analysis shows that both primary and cultured cells express the plasma-cell-associated antigens PCA-1 and T10, while specific B- and T-cell determinants and EBV nuclear antigen are undetectable. In the established cell line a few cells express Ia-like and CALLA antigens. Cytogenetic analysis of MT3 cells reveals a prevalent hypertriploid karyotype with constant chromosomal aberrations consisting of 14q+, 22q- and marker chromosomes.
我们从一名患有IgA-κ浆细胞白血病患者的外周血中建立了一种新的人浆细胞系。形态学、免疫学、细胞遗传学和分子学研究证实，培养的细胞源自体内白血病浆细胞的同一克隆。建立的细胞系（MT3）悬浮生长，分泌大量IgA κ，并表现出浆细胞的形态和超微结构特征。表面标志物分析显示，原代细胞和培养细胞均表达浆细胞相关抗原PCA-1和T10，而未检测到特异性B细胞和T细胞决定簇以及EBV核抗原。在建立的细胞系中，少数细胞表达Ia样和CALLA抗原。对MT3细胞的细胞遗传学分析显示，其普遍为超三倍体核型，具有由14q+、22q-和标记染色体组成的恒定染色体畸变。
