Muñoz-Leal Sebastián, Terassini Flavio A, Marcili Arlei, Oliveira Glauber M B, Camargo Luís Marcelo A, Labruna Marcelo B
Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, Cidade Universitária, São Paulo, SP, 05508-270, Brazil.
São Lucas Educacional, Rua Alexandre Guimarães, No. 1927 Bairro, Areal, Porto Velho, RO, Brazil.
Syst Parasitol. 2019 Sep;96(7):595-602. doi: 10.1007/s11230-019-09873-9. Epub 2019 Jul 31.
In South America, early descriptions of soft tick species were based on examination of the external morphology of the larval stages. In many cases, specimens were collected only once as it is the case of some bat-associated Ornithodoros spp. If we are to understand the systematic scenario of South American soft ticks, these species become axial questions to be re-studied from a morphological and molecular point of view. The objective of this study was to assess the taxonomic identity of soft tick larvae collected on bats inhabiting crevices of a large rock in the Rondônia State (RO), Brazilian Amazon. After a detailed morphological analysis using light microscopy, three large engorged larvae sharing the same phenotype were identified as Ornithodoros setosus Kohls, Clifford & Jones, 1969, a species collected in 1964 on bats in RO. Remarkably, maximum parsimony and Bayesian phylogenetic analyses based on tick 16S rRNA mitochondrial gene sequences obtained from two of these specimens showed that O. setosus indeed corresponds to a species of Nothoaspis Keirans & Clifford, 1975. Therefore, a new combination, Nothoaspis setosus (Kohls, Clifford & Jones, 1969), is herein proposed. While an elongated triangular dorsal plate with a curvy-notched posterior margin, and bulges in the lateral margins of basis capitulum correspond to common characters in larvae of the genus Nothoaspis, polymorphic traits are represented by minute cornua in the basis of the capitulum, the dentition of the hypostomal tip, triangular spurs on coxae I, and the number of dorsal and circumanal setae.
在南美洲，对软蜱物种的早期描述基于对幼虫阶段外部形态的检查。在许多情况下，标本只采集过一次，一些与蝙蝠相关的钝缘蜱属物种就是如此。如果我们要了解南美洲软蜱的系统情况，就需要从形态学和分子学角度重新研究这些物种，这成为核心问题。本研究的目的是评估在巴西亚马逊朗多尼亚州（RO）一块大石头裂缝中栖息的蝙蝠身上采集到的软蜱幼虫的分类身份。在使用光学显微镜进行详细的形态学分析后，三只具有相同表型的饱血大幼虫被鉴定为1969年由科尔兹、克利福德和琼斯命名的刺钝缘蜱，该物种于1964年在朗多尼亚州的蝙蝠身上采集到。值得注意的是，基于从其中两个标本获得的蜱16S rRNA线粒体基因序列进行的最大简约法和贝叶斯系统发育分析表明，刺钝缘蜱实际上属于1975年基兰斯和克利福德命名的伪锐缘蜱属物种。因此，本文提出一个新组合，即伪锐缘蜱（科尔兹、克利福德和琼斯，1969年）。虽然具弯曲缺刻后缘的细长三角形背板以及假头基部侧缘的凸起是伪锐缘蜱属幼虫的共同特征，但多态性特征表现为假头基部的微小角突、口下板顶端的齿列、第一基节上的三角形距以及背毛和肛周毛的数量。
