School of Chemistry and Molecular Engineering, Shanghai Key Laboratory for Urban Ecological Processes and Eco-Restoration , East China Normal University , 500 Dongchuan Road , Shanghai 200241 , China.
Joint Research Center for Precision Medicine, School of Chemistry and Chemical Engineering and School of Medicine, Sixth People's Hospital South Campus , Shanghai Jiao Tong University , Shanghai 200240 , China.
Anal Chem. 2019 Sep 3;91(17):11170-11177. doi: 10.1021/acs.analchem.9b01879. Epub 2019 Aug 15.
A protein/lanthanide complex (BSA/Tb)-based sensor array in two different pH buffers has been designed for high-throughput recognition and time-resolved fluorescence (TRF) detection of metal ions in biofluids. BSA, which acted as an antenna ligand, can sensitize the fluorescence of Tb (i.e., antenna effect), while the presence of metal ions would lead to the corresponding conformational change of BSA for altering the antenna effect accompanied by a substantial TRF performance of Tb. This principle has also been fully proved by both experimental characterizations and coarse-grained molecular dynamics (CG-MD) studies. By using Tris-HCl buffer with different pHs (at 7.4 and 8.5), 17 metal ions have been well-distinguished by using our proposed BSA/Tb sensor array. Moreover, the sensor array has the potential to discriminate different concentrations of the same metal ions and a mixture of metal ions. Remarkably, the detection of metal ions in biofluids can be realized by utilizing the presented sensor array, verifying its practical applications. The platform avoids the synthesis of multiplex sensing receptors, providing a new method for the construction of convenient and feasible lanthanide complex-based TRF sensing arrays.
已设计了一种基于蛋白质/镧系元素配合物（BSA/Tb）的传感器阵列，在两种不同 pH 缓冲液中用于高通量识别和时间分辨荧光（TRF）检测生物流体中的金属离子。BSA 作为天线配体，可以敏化 Tb 的荧光（即天线效应），而金属离子的存在会导致 BSA 发生相应的构象变化，从而改变天线效应，并伴随着 Tb 的显著 TRF 性能变化。这一原理也通过实验表征和粗粒化分子动力学（CG-MD）研究得到了充分证明。通过使用具有不同 pH 值的 Tris-HCl 缓冲液（分别为 7.4 和 8.5），我们提出的 BSA/Tb 传感器阵列可以很好地区分 17 种金属离子。此外，该传感器阵列还有潜力区分相同金属离子的不同浓度和金属离子的混合物。值得注意的是，通过利用所提出的传感器阵列可以实现对生物流体中金属离子的检测，验证了其实际应用。该平台避免了多传感受体的合成，为构建方便可行的镧系元素配合物 TRF 传感阵列提供了一种新方法。
