Student Research Committee, Babol University of Medical sciences, Babol, Iran.
Dental Materials Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
J Food Biochem. 2019 Aug;43(8):e12926. doi: 10.1111/jfbc.12926. Epub 2019 Jun 10.
Propolis had a wide spectrum of biological activities. In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated. Bioactive composition of the PEEP was determined by HPLC analysis. Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated. HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid. The PEEP inhibited the proliferation of RAW 264.7 cells with IC 15 ± 3.2 µg/ml. Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP. Additionally, expression of Cox-2, IL-1β and IL-6 significantly decreased. The obtained results supported the PEEP anti-inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX-2, IL-1β, and IL-6 expression. PRACTICAL APPLICATIONS: Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times. The main compounds found in propolis are typically various and depend on the type of plants and climatic region. In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti-inflammatory, as well as antifungal properties. This extraordinary substance is rich in flavonoids and antioxidants. Therefore, it is now widely used in foods and drinks with the claim that it can maintain or improve human health.
蜂胶具有广泛的生物活性。在本研究中，研究了蜂胶的 Polur 乙醇提取物（PEEP）对小鼠巨噬细胞（RAW 264.7）的抗氧化和免疫调节作用。通过 HPLC 分析确定了 PEEP 的生物活性成分。用不同浓度的 PEEP 和 LPS 处理细胞，然后评估细胞活力、NO 水平和炎症因子的表达。PEEP 的 HPLC 分析表明存在类黄酮和酚酸。PEEP 抑制 RAW 264.7 细胞的增殖，IC 15 ± 3.2 µg/ml。0.15 µg/ml 的 PEEP 可显著减少活性氧（ROS）和 NO 的产生。此外，Cox-2、IL-1β 和 IL-6 的表达明显降低。研究结果表明，PEEP 对 RAW 264.7 细胞的抗炎作用可能是通过降低 ROS 和 NO 产生以及 COX-2、IL-1β 和 IL-6 的表达来实现的。
蜂胶是蜜蜂产生的一种树脂状物质，自古以来就被用作传统药物。蜂胶中的主要化合物通常各不相同，取决于植物的种类和气候区域。在这方面，已经确定了蜂胶具有广泛的生物活性，包括抗氧化、抗菌、抗癌、抗炎和抗真菌特性。这种特殊的物质富含类黄酮和抗氧化剂。因此，它现在广泛用于食品和饮料中，声称可以维持或改善人类健康。
