Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, People's Republic of China.
Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, People's Republic of China.
J Food Biochem. 2019 Aug;43(8):e12901. doi: 10.1111/jfbc.12901. Epub 2019 May 29.
In the study, six adventitious root lines of Panax ginseng have been successfully established. HPLC-ESI-MS analysis showed that 20 ginsenosides were identified in root lines, notoginsenoside Fa and notoginsenoside R were not found in AR lines. In AR lines, the highest accumulation of total ginsenosides was obtained in five-year main AR (24.87 mg/g). Principal component analysis classified root lines into three groups. Five-year ginseng was mostly similar with five-year main AR, five-year rootlet AR, and four-year rootlet AR in ginsenosides composition of group 1. Besides, gene expressions were consistent with the production of total ginsenosides, and correlation analysis revealed that total ginsenosides biosynthesis was significantly positively correlated with the gene expression of dammarenediol synthase. Five-year rootlet AR showed the highest activity on ferric-reducing antioxidant power test among samples. It provides a scientific evidence for the further exploitation and large-scale production of P. ginseng. PRACTICAL APPLICATIONS: This study provides valuable information for the commercial scale culture of ginseng adventitious roots. This report combines morphology, ginsenoside composition and content, gene expression, and ferric-reducing antioxidant power test to evaluate the quality of P. ginseng adventitious root, and combined with principal component analysis to screen out the high yield and stable ginseng adventitious roots. It would be profitable to use adventitious root culture of P. ginseng instead of field cultivation.
在这项研究中，成功建立了 6 条人参不定根系。HPLC-ESI-MS 分析表明，在根系中鉴定出了 20 种人参皂苷，而在 AR 系中未发现三七皂苷 Fa 和三七皂苷 R。在 AR 系中，五年主 AR（24.87mg/g）中总人参皂苷的积累最高。主成分分析将根系分为三组。五年生人参与五年主 AR、五年生根 AR 和四年生根 AR 在第一组的人参皂苷组成上最为相似。此外，基因表达与总人参皂苷的产生一致，相关性分析表明，总人参皂苷的生物合成与达玛烯二醇合酶的基因表达呈显著正相关。在铁还原抗氧化能力测试中，五年生根 AR 表现出最高的活性。为进一步开发和大规模生产人参提供了科学依据。
本研究为人参不定根的商业规模培养提供了有价值的信息。本报告结合形态学、人参皂苷组成和含量、基因表达和铁还原抗氧化能力测试来评价人参不定根的质量，并结合主成分分析筛选出高产、稳定的人参不定根。用人参不定根培养代替田间栽培将有利可图。
