Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, China.
Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, China.
J Food Biochem. 2019 Apr;43(4):e12794. doi: 10.1111/jfbc.12794. Epub 2019 Feb 1.
This study researched the effect of temperature on growth and ginsenosides accumulation in adventitious root cultures of Panax ginseng. Results showed that the ginseng adventitious roots growth and differentiation ability could be affected faced with different incubation temperatures (15, 20, 25, and 30°C for 35 days). Besides, the research also demonstrated that low-temperature stimulation could promote the accumulation of ginsenosides and the content of total ginsenosides increased by 2.53 times at 10°C-7d (10°C for 7 days and then transferred to 25°C for 28 days) compared with that at 25°C. Moreover, the transcriptional levels of functional genes and PgWRKYs were analyzed by this study and the correlation analysis showed that GPS, SS, CYP716A47, CYP716A53v2, UGT74AE2, UGT94Q2, PgWRKY1, PgWRKY3, and PgWRKY8 were significantly correlated with total ginsenosides content. Furthermore, HPLC-ESI-MS analyzed that Malonyl-Rb only existed in 10°C-7d group. PRACTICAL APPLICATIONS: The survey showed that after a certain time of stimulating P. ginseng adventitious roots at low temperature, the accumulation of ginsenosides could be enhanced as their expression of related genes were regulated. It provides a theoretical foundation for the mass production of ginsenosides by controlling the temperature conditions of P. ginseng adventitious roots.
本研究探讨了温度对人参不定根培养中生长和人参皂苷积累的影响。结果表明，不同的培养温度（15、20、25 和 30°C，35 天）会影响人参不定根的生长和分化能力。此外，研究还表明，低温刺激可以促进人参皂苷的积累，与 25°C 相比，10°C-7d（10°C 培养 7 天，然后转移到 25°C 培养 28 天）时总人参皂苷含量增加了 2.53 倍。此外，本研究还通过分析功能基因和 PgWRKYs 的转录水平，相关性分析表明，GPS、SS、CYP716A47、CYP716A53v2、UGT74AE2、UGT94Q2、PgWRKY1、PgWRKY3 和 PgWRKY8 与总人参皂苷含量显著相关。此外，通过 HPLC-ESI-MS 分析发现，只有在 10°C-7d 组中存在丙二酰-Rb。实际应用：研究表明，在低温刺激人参不定根一定时间后，通过调节相关基因的表达，可以增强人参皂苷的积累。这为通过控制人参不定根的温度条件来大规模生产人参皂苷提供了理论基础。
