Johnson G V, Jope R S
Department of Pharmacology, University of Alabama, Birmingham 35294.
Brain Res. 1988 Jul 19;456(1):95-103. doi: 10.1016/0006-8993(88)90350-2.
In rats, administration of 0.3% aluminum in the drinking water for 4-5 weeks significantly increased the in vivo incorporation of 32-phosphorous (32Pi) into proteins with apparent molecular weights of 300 and 210 kDa in the brainstem and cerebral cortex. The identities of these two phosphoproteins as microtubule-associated protein-2 (MAP-2) and the 200 kDa neurofilament subunit (NF), respectively, were established using immunoprecipitation techniques with monoclonal antibodies. Aluminum treatment did not significantly change the amount of MAP-2 or 200 kDa NF in the cerebral cortex and brainstem. Phosphorylation of MAP-2 in aluminum-treated rats in the brainstem and cerebral cortex was 163 and 155% of control values, respectively. The phosphorylation of 200 kDa NF in the brainstem and cerebral cortex of aluminum-treated rats was 148 and 209% of control values, respectively. These results demonstrate that chronic oral aluminum administration to rats increases the phosphorylation of certain cytoskeletal proteins. This treatment regimen may provide a model system with which the mechanisms and consequences of altered in vivo phosphorylation of cytoskeletal proteins can be studied.
在大鼠中，饮用含0.3%铝的水4 - 5周后，可显著增加32 - 磷（32Pi）在脑干和大脑皮层中体内掺入表观分子量为300 kDa和210 kDa的蛋白质中的量。使用单克隆抗体的免疫沉淀技术确定这两种磷蛋白分别为微管相关蛋白-2（MAP-2）和200 kDa神经丝亚基（NF）。铝处理并未显著改变大脑皮层和脑干中MAP-2或200 kDa NF的量。在铝处理的大鼠中，脑干和大脑皮层中MAP-2的磷酸化分别为对照值的163%和155%。铝处理的大鼠脑干和大脑皮层中200 kDa NF的磷酸化分别为对照值的148%和209%。这些结果表明，对大鼠长期口服铝可增加某些细胞骨架蛋白的磷酸化。这种处理方案可能提供一个模型系统，用以研究体内细胞骨架蛋白磷酸化改变的机制和后果。
