a Department of Orthopedic, Weifang Yidu Central Hospital , Weifang , Shandong , China.
b Department of Orthopedic, Weifang People's Hospital , Weifang , China.
Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):3259-3264. doi: 10.1080/21691401.2019.1623227.
Impairment of type II collagen caused by MMPs in response to overproduction of IL-1β is an important step in the pathological progression of osteoarthritis (OA). Lunasin, a well-known peptide present in the soybean, has displayed a positive impact on numerous physiological functions. Little information in the effects of lunasin on cartilage degradation has been sought in clinical research before. Here, we report that lunasin suppressed the increase in MMP-3 and MMP-13 caused by IL-1β. In addition, we found that lunasin could prevent the decrease in TIMP-1 and TIMP-2 expressions caused by IL-1β. Notably, lunasin suppressed reduction of type II collagen, the basis for articular cartilage. Lunasin also attenuated activation of the JAK2/STAT1/IRF-1 pathway. These effects of lunasin suggest that it might become a promising therapeutic agent for chondro-protective therapy.
基质金属蛋白酶（MMPs）对 II 型胶原蛋白的损伤，以及白细胞介素-1β（IL-1β）的过度产生，是骨关节炎（OA）病理进展的重要步骤。亮氨酸脑啡肽，一种存在于大豆中的著名肽，对许多生理功能都有积极的影响。在临床研究中，人们之前很少关注亮氨酸脑啡肽对软骨降解的影响。在这里，我们报告亮氨酸脑啡肽抑制了 IL-1β引起的 MMP-3 和 MMP-13 的增加。此外，我们发现亮氨酸脑啡肽可以防止 IL-1β引起的 TIMP-1 和 TIMP-2 表达的减少。值得注意的是，亮氨酸脑啡肽抑制了 II 型胶原蛋白的减少，而 II 型胶原蛋白是关节软骨的基础。亮氨酸脑啡肽还能减弱 JAK2/STAT1/IRF-1 通路的激活。亮氨酸脑啡肽的这些作用表明，它可能成为一种有前途的软骨保护治疗剂。
