星形胶质细胞中甘油二酯模拟物对受体介导信号转导的调节作用
Modulation of receptor-mediated signal transduction by diacylglycerol mimetics in astrocytes.
作者信息
Burch R M, Kniss D A
机构信息
Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland 20892.
出版信息
Cell Mol Neurobiol. 1988 Jun;8(2):251-7. doi: 10.1007/BF00711251.
Abstract
- When rat astrocytes in primary culture were incubated with bradykinin, inositol phosphate formation and arachidonic acid release were stimulated. 2. By themselves, phorbol esters inhibited inositol phosphate formation, but phorbol esters and other cell-permeant diacylglycerol analogues stimulated arachidonic acid release. Preincubation of the cells with phorbol esters or diacylglycerol analogues blocked bradykinin-stimulated inositol phosphate formation but augmented bradykinin-stimulated arachidonic acid release. 3. The present results suggest that, in astrocytes, bradykinin activates at least two signal transduction pathways bradykinin stimulates a phosphatidylinositol-specific phospholipase C leading to enhanced inositol phosphate formation, and bradykinin stimulates a second phospholipase to enhance arachidonic acid release. The pathways may be distinguished using phorbol esters and other diacylglycerol mimetics. 4. The possibility is raised that diacylglycerol, formed in response to bradykinin, may serve as a transducer of receptor-receptor interactions by altering the ability of receptors to stimulate phospholipase activity.
摘要
- 当原代培养的大鼠星形胶质细胞与缓激肽一起孵育时，肌醇磷酸的形成和花生四烯酸的释放受到刺激。2. 佛波酯本身会抑制肌醇磷酸的形成，但佛波酯和其他可透过细胞的二酰基甘油类似物会刺激花生四烯酸的释放。用佛波酯或二酰基甘油类似物对细胞进行预孵育会阻断缓激肽刺激的肌醇磷酸形成，但会增强缓激肽刺激的花生四烯酸释放。3. 目前的结果表明，在星形胶质细胞中，缓激肽激活至少两条信号转导途径，缓激肽刺激磷脂酰肌醇特异性磷脂酶C导致肌醇磷酸形成增加，缓激肽刺激第二种磷脂酶以增强花生四烯酸释放。可以使用佛波酯和其他二酰基甘油模拟物来区分这些途径。4. 由此提出一种可能性，即缓激肽刺激产生的二酰基甘油可能通过改变受体刺激磷脂酶活性的能力，作为受体-受体相互作用的转导分子。
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