McCormack J M, Cunningham M W
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Cell Immunol. 1988 Sep;115(2):325-33. doi: 10.1016/0008-8749(88)90185-2.
We studied the effect of highly purified recombinant interleukin 2 from Escherichia coli (rIL-2) on antibody production by our human hybridomas. Increasing concentrations of rIL-2 were directly related to increased production of immunoglobulin, which reached peak levels of 13-25 micrograms/ml, a 10- to 20-fold increase over untreated hybridomas. We were unable to demonstrate large quantities of the IL-2 receptor on the hybridomas as measured by an anti-TAC monoclonal antibody. The data suggest that the antibody enhancement phenomenon is mediated by IL-2 and that is a new, effective way to achieve higher levels of immunoglobulin from human hybridomas.
我们研究了来自大肠杆菌的高度纯化的重组白细胞介素2（rIL-2）对我们的人杂交瘤抗体产生的影响。rIL-2浓度的增加与免疫球蛋白产量的增加直接相关，免疫球蛋白产量达到13 - 25微克/毫升的峰值水平，比未处理的杂交瘤增加了10至20倍。通过抗TAC单克隆抗体检测，我们无法在杂交瘤上证明大量的IL-2受体。数据表明，抗体增强现象是由IL-2介导的，这是一种从人杂交瘤获得更高水平免疫球蛋白的新的有效方法。
