Iwamoto K, Shintani Y, Sasada R, Honjo T, Kitano K
Microbiology Research Laboratories, Takeda Chemical Industries, Ltd., Osaka, Japan.
Cytotechnology. 1990 Jul;4(1):29-37. doi: 10.1007/BF00148808.
The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5-2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor alpha) antibody. To enhance the response of the hybridoma cells to rIL-2, Tac gene was introduced by co-transfection with Tac gene expression plasmid pTB459 and G418 resistant gene expression plasmid pRSVneo. Tac cDNA transfected hybridoma (HBW-4.16.459-6-126) was induced to produce 6 times as much IgG by rIL-2 as was the control. This antibody production promoting phenomenon mediated by rIL-2 was depressed by anti-Tac antibody.
高纯度重组白细胞介素-2（rIL-2）的存在使人-人杂交瘤产生免疫球蛋白（IgM或IgG）的量增加至未处理细胞产量的1.5 - 2.0倍。然而，这些细胞不与抗Tac（IL-2受体α）抗体发生反应。为增强杂交瘤细胞对rIL-2的反应，通过与Tac基因表达质粒pTB459和G418抗性基因表达质粒pRSVneo共转染来导入Tac基因。转染Tac cDNA的杂交瘤（HBW-4.16.459-6-126）被rIL-2诱导产生的IgG量是对照的6倍。由rIL-2介导的这种抗体产生促进现象被抗Tac抗体所抑制。
