Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Department of Histology and Embryology, Xuzhou Medical University, Xuzhou, China.
Microb Drug Resist. 2019 Dec;25(10):1465-1474. doi: 10.1089/mdr.2018.0229. Epub 2019 Aug 1.
Emergence of multidrug-resistant , a major causative agent of bacterial dysentery, has generated many concerns not only in China but also worldwide. However, the prevalence of resistance caused by integron in the nonpopular season of diarrhea is not clear. Thirty-one and 22 samples collected in December 2010 from 10 cities of China were characterized for antimicrobial susceptibility, gene cassettes, widespread of integrons, and pulsed-field gel electrophoresis (PFGE) profile. Multidrug resistance (MDR) was detected in 29 (93.5%) and 20 (90.9%) isolates. Class 1 integrons were detected in 25 (80.6%) and in 13 (59.1%) isolates; class 2 integrons were detected in 26 (83.9%) and in 19 (86.4%) isolates. Interestingly, the atypical class 1 integrons were mostly detected in (45.2%) isolates, whereas in only 1 (4.5%) isolate. DNA sequencing revealed two novel cassette arrays, and , of class 1 integrons in , and in isolates. The cassette arrays, of class 2 integron and - of atypical class 1 integron, were also identified. PFGE profiles demonstrated A6 subtype of strains prevalent in Shanghai, Changchun, Jinan, and Changsha; and F6 subtype of prevalent in Jinan, Changchun, and Shanghai. The dissemination of MDR strains with integrons makes it an increasing public health problem in China. Increased surveillance and the development of adequate prevention strategies are warranted.
多重耐药 ，一种主要的细菌性痢疾病原体，不仅在中国，而且在世界范围内都引起了许多关注。然而，在腹泻非流行季节，整合子引起的耐药性的流行情况尚不清楚。我们对 2010 年 12 月从中国 10 个城市采集的 31 株和 22 株 进行了抗菌药敏试验、基因盒、整合子广泛分布和脉冲场凝胶电泳（PFGE）分析。29 株（93.5%）和 20 株（90.9%）分离株检测到多重耐药性（MDR）。25 株（80.6%）和 13 株（59.1%）分离株检测到 1 类整合子；26 株（83.9%）和 19 株（86.4%）分离株检测到 2 类整合子。有趣的是，非典型 1 类整合子主要存在于 （45.2%）株，而在 株中仅发现 1 株（4.5%）。DNA 测序揭示了 株 中 1 类整合子的两个新的盒式数组 和 ，以及 株 中 2 类整合子的盒式数组 和非典型 1 类整合子的 。还鉴定了 2 类整合子的盒式数组 和非典型 1 类整合子的 。PFGE 图谱显示，上海、长春、济南和长沙流行的 株为 A6 亚型；济南、长春和上海流行的 株为 F6 亚型。携带整合子的多药耐药 株的传播使它成为中国日益严重的公共卫生问题。需要加强监测并制定适当的预防策略。
