使用回收的 [BMIM]Cl 从湿的蛋白核小球藻污泥中提取脂质。
Lipids extraction from wet Chlorella pyrenoidosa sludge using recycled [BMIM]Cl.
机构信息
Key Laboratory of Safety Science of Pressurized System (MOE), School of Mechanical Engineering, East China University of Science and Technology, Shanghai 200237, China.
Key Laboratory of Safety Science of Pressurized System (MOE), School of Mechanical Engineering, East China University of Science and Technology, Shanghai 200237, China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Bioresour Technol. 2019 Nov;291:121819. doi: 10.1016/j.biortech.2019.121819. Epub 2019 Jul 16.
In this study, experiments on pretreating one species of microalgae (Chlorella pyrenoidosa) using one kind of ionic liquid (IL) of [BMIM]Cl were conducted. The aim of this work is to evaluate the recycling efficacy of expensive IL solvent for effective cell disruption. It was indicated that the molecular structure of IL was stable during the recycling test. Five times antisolvent precipitation of microalgae debris after lipid extraction using methanol recovered 99.8% IL with the energy consumption of 4.46 MJ per kg dry Chlorella pyrenoidosa. The chromatography was used to separate IL and hydrolysates, resulting in the IL loss below 1.97 g per kg dry Chlorella pyrenoidosa.
本研究使用一种离子液体 ([BMIM]Cl) 对一种微藻（椭圆小球藻）进行预处理实验。本工作旨在评估昂贵的离子液体溶剂对有效细胞破碎的回收效果。结果表明，在回收测试中，离子液体的分子结构稳定。使用甲醇进行脂质提取后，通过五次抗溶剂沉淀微藻残渣，可回收 99.8%的离子液体，能量消耗为每公斤干燥椭圆小球藻 4.46 兆焦耳。通过色谱法分离离子液体和水解产物，导致每公斤干燥椭圆小球藻的离子液体损失低于 1.97 克。
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