蛋白质与基于偶氮苯的交联剂的分子内交联。

Intramolecular cross-linking of proteins with azobenzene-based cross-linkers.

作者信息

Yasuike Nobuo, Lu Huixin, Xia Peng, Woolley G Andrew

机构信息

University of Toronto, Department of Chemistry, Toronto, ON, Canada; JSR Corporation, Tokyo, Japan.

University of Toronto, Department of Chemistry, Toronto, ON, Canada.

出版信息

Methods Enzymol. 2019;624:129-149. doi: 10.1016/bs.mie.2019.04.006. Epub 2019 Apr 25.

DOI:10.1016/bs.mie.2019.04.006

PMID:31370927

Abstract

The photo-control of protein activity can often be achieved via the photo-control of protein structure. Intramolecular cross-linkers that change length upon photoisomerization provide a means to photo-control protein structure by linking to pairs of Cys residues in a protein sequence. In this protocol, we describe general methods for introducing intramolecular cross-linkers, both UV light switchable and red-light switchable, under either denaturing or native conditions.

摘要

蛋白质活性的光控通常可通过蛋白质结构的光控来实现。光异构化时长度发生变化的分子内交联剂提供了一种通过与蛋白质序列中的半胱氨酸残基对相连来光控蛋白质结构的方法。在本实验方案中，我们描述了在变性或天然条件下引入分子内交联剂（包括紫外线可切换和红光可切换的交联剂）的一般方法。