Matsushita T, Hirata H, Kusaka I
Institute of Applied Microbiology, University of Tokyo, Japan.
FEBS Lett. 1988 Aug 29;236(2):437-40. doi: 10.1016/0014-5793(88)80072-3.
The effect of several Ca2+ channel blockers, which inhibit the voltage-dependent Ca2+ uptake in Bacillus subtilis, on chemotactic behaviour of the bacterium was studied. Nitrendipine, verapamil, LaCl3 and omega-conotoxin were tested and these blockers inhibited chemotactic behaviour in the bacterium toward L-alanine. Among these blockers, omega-conotoxin was the most effective inhibitor of chemotaxis. EGTA was also as effective as omega-conotoxin. In contrast, these blockers, did not inhibit the motility and the growth of the bacterium. These results suggest that internal Ca2+ plays an important role in the sensory system of bacterial chemotaxis.
研究了几种抑制枯草芽孢杆菌中电压依赖性钙摄取的钙通道阻滞剂对该细菌趋化行为的影响。对尼群地平、维拉帕米、氯化镧和ω-芋螺毒素进行了测试，这些阻滞剂抑制了细菌对L-丙氨酸的趋化行为。在这些阻滞剂中，ω-芋螺毒素是最有效的趋化抑制剂。乙二醇双四乙酸（EGTA）的效果也与ω-芋螺毒素相当。相比之下，这些阻滞剂并不抑制细菌的运动性和生长。这些结果表明，细胞内钙在细菌趋化感觉系统中起重要作用。
