Seidler U, Knafla K, Kownatzki R, Sewing K F
Abteilungen Allgemeine Pharmakologie und Medizinische Messgeraete, Medizinische Hochschule, Hannover, Federal Republic of Germany.
Gastroenterology. 1988 Oct;95(4):945-51. doi: 10.1016/0016-5085(88)90167-9.
We studied gastric glycoprotein synthesis and secretion in organ culture before and during cyclooxygenase inhibition and replacement with exogenous prostaglandins (16,16-dimethyl prostaglandin E2 and prostaglandin F2 alpha). Isolated rabbit antral and fundic mucosal explants incorporated [14C]N-acetylglucosamine and [3H]leucine in a linear fashion and steadily secreted labeled proteins and glycoproteins during the 24-h incubation period. On sepharose 4B, greater than 90% of the secreted protein-bound [14C]N-acetylglucosamine was found in the high molecular weight peak. Incorporation of tracer was not influenced by cyclooxygenase inhibition with indomethacin or the addition of exogenous prostaglandins. Secretion of newly formed glycoprotein, however, was significantly inhibited by indomethacin and stimulated by both tested prostaglandins in a concentration-dependent manner. 16,16-Dimethyl prostaglandin E2 caused significant stimulation in concentrations that are well in the physiologic range for endogenous prostaglandin E2, whereas prostaglandin F2 alpha stimulated in 100 times higher concentrations. We conclude that in the isolated gastric mucosa both endogenous and exogenous prostaglandins stimulate mucus secretion. For prostaglandin E2, but not prostaglandin F2 alpha, a role in the physiologic regulation of gastric mucus secretion is probable.
我们在环氧化酶抑制以及用外源性前列腺素（16,16 - 二甲基前列腺素E2和前列腺素F2α）替代的前后阶段，对器官培养中的胃糖蛋白合成与分泌进行了研究。分离的兔胃窦和胃底黏膜外植体以线性方式掺入[14C]N - 乙酰葡糖胺和[3H]亮氨酸，并在24小时孵育期内稳定分泌标记的蛋白质和糖蛋白。在琼脂糖4B上，超过90%的分泌型蛋白结合[14C]N - 乙酰葡糖胺存在于高分子量峰中。示踪剂的掺入不受吲哚美辛对环氧化酶的抑制或外源性前列腺素添加的影响。然而，吲哚美辛显著抑制新形成糖蛋白的分泌，而两种受试前列腺素均以浓度依赖性方式刺激其分泌。16,16 - 二甲基前列腺素E2在处于内源性前列腺素E2生理范围内的浓度时即可引起显著刺激，而前列腺素F2α则需在高100倍的浓度下才产生刺激作用。我们得出结论，在分离的胃黏膜中，内源性和外源性前列腺素均刺激黏液分泌。对于前列腺素E2而言，在胃黏液分泌的生理调节中可能发挥作用，但前列腺素F2α并非如此。
