腹腔内免疫后，腹水中可特异性诱导出针对二硝基苯酚（DNP）的IgD抗体。
IgD antibodies to DNP are specifically induced in ascitic fluid after intraperitoneal immunization.
作者信息
Steele M G, Leslie G A
机构信息
Department of Microbiology and Immunology, Oregon Health Sciences University, Portland.
出版信息
Immunology. 1988 Jul;64(3):391-5.
The induction characteristics of IgD in serum and other body fluids are poorly understood. Here we report that Freund's complete adjuvant (FCA), when used in intraperitoneal (i.p.) immunization, resulted in significantly higher levels of IgD and specific IgD antibodies in ascitic fluid than in the serum of the same animals. This was in sharp contrast to the distribution of IgM and kappa light chain-bearing immunoglobulins (kappa Ig) between these compartments. Female rats were immunized subcutaneously (s.c.) on Day 0 with 1:1 FCA mixed with either 2,4 dinitrophenyl group A streptococcal vaccine (DNP-GASV), 2,4 dinitrophenyl ovalbumin (DNP-OVA), or saline. A week later (and weekly thereafter) they were boosted i.p. with these antigens mixed with FCA 1:9. Eleven of sixteen rats produced some ascites by Day 21, and 15/16 by Day 28. Total immunoglobulin and specific antibodies for the heavy-chain isotypes IgD and IgM and for kappa Ig were measured by ELISA. Between immunization groups no significant differences were observed in total immunoglobulin levels. Subcutaneous immunization with 1:1 FCA caused a significant two- to six-fold increase in serum IgD by Day 7 (P less than 0.01), while no change occurred with serum IgM or kappa Ig. Ascitic fluid collected on Day 21 had significantly high levels of IgD than that of serum IgD (P less than 0.01), with a mean level nine times that of the serum. In contrast, levels of total IgM, kappa Ig and total protein were significantly lower in ascitic fluid than in serum (all P less than 0.05). The high levels of IgD in ascitic fluid disappeared in Day 28 ascitic fluid, while IgM and kappa Ig levels were relatively unchanged. The ratio of ascitic fluid to serum IgD anti-DNP was 9.8 for the DNP-GASV-immunized group on Day 21, and 23.9 for the DNP-OVA-immunized group on day 28. The same ratios for IgM anti-DNP and kappa Ig anti-DNP were much lower, ranging from 0.4 to 2.3 and 0.3 to 3.3, respectively.
血清及其他体液中IgD的诱导特性目前了解甚少。在此我们报告，弗氏完全佐剂（FCA）用于腹腔内（i.p.）免疫时，与相同动物血清相比，腹水中的IgD及特异性IgD抗体水平显著更高。这与这些区室中IgM及携带κ轻链的免疫球蛋白（κ Ig）的分布形成鲜明对比。雌性大鼠于第0天皮下（s.c.）注射1:1 FCA与2,4-二硝基苯基A群链球菌疫苗（DNP-GASV）、2,4-二硝基苯基卵清蛋白（DNP-OVA）或生理盐水的混合物。一周后（及此后每周），它们腹腔内注射这些抗原与1:9 FCA的混合物进行加强免疫。到第21天，16只大鼠中有11只产生了一些腹水，到第28天，16只中有15只产生了腹水。通过ELISA检测总免疫球蛋白以及重链同种型IgD和IgM及κ Ig的特异性抗体。免疫组之间在总免疫球蛋白水平上未观察到显著差异。第7天时，皮下注射1:1 FCA使血清IgD显著升高2至6倍（P小于0.01），而血清IgM或κ Ig无变化。第21天收集的腹水IgD水平显著高于血清IgD（P小于0.01），平均水平是血清的9倍。相比之下，腹水的总IgM、κ Ig和总蛋白水平显著低于血清（均P小于0.05）。腹水中高水平的IgD在第28天的腹水中消失，而IgM和κ Ig水平相对未变。第21天，DNP-GASV免疫组腹水与血清IgD抗DNP的比值为9.8，第28天，DNP-OVA免疫组该比值为23.9。IgM抗DNP和κ Ig抗DNP的相同比值则低得多，分别为0.4至2.3和0.3至3.3。
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