Richardson W P, Sadoff J C
Department of Bacterial Disease, Walter Reed Army Institute, Washington, D.C. 20307.
Infect Immun. 1988 Sep;56(9):2512-4. doi: 10.1128/iai.56.9.2512-2514.1988.
Engulfment of gonococci by mammalian tissue culture cells was examined as a model of the penetration of host cells in gonorrhea. Engulfment required viable organisms; killing the gonococci with heat or refrigeration abolished the process. Engulfment also required tissue culture cell microtubule- and microfilament-dependent movement; treating the cells with cytochalasin B (0.5 micrograms/ml) or demecolcine (Colcemid; Ciba-Geigy AG, Basel, Switzerland) (10 micrograms/ml) also prevented his process.
作为淋病中宿主细胞穿透模型，研究了哺乳动物组织培养细胞对淋球菌的吞噬作用。吞噬作用需要活的生物体；加热或冷藏杀死淋球菌会使该过程消失。吞噬作用还需要组织培养细胞微管和微丝依赖性运动；用细胞松弛素B（0.5微克/毫升）或秋水仙酰胺（秋水仙碱；瑞士巴塞尔汽巴-嘉基公司）（10微克/毫升）处理细胞也会阻止这一过程。
