Carl M, Ching W M, Dasch G A
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20814-5055.
Infect Immun. 1988 Sep;56(9):2526-9. doi: 10.1128/iai.56.9.2526-2529.1988.
Lymphokine-activated killer cells caused significant lysis of target cells infected with typhus group rickettsiae (TGR) but not cells infected with Rickettsia tsutsugamushi. Monoclonal and polyclonal antibodies against TGR or TGR-infected cells failed to bind specifically to infected targets, as determined by flow cytometry or by immunoprecipitation of radiolabeled cell surface proteins. Major unaltered antigenic determinants of TGR therefore cannot be detected on the surface of infected targets.
淋巴因子激活的杀伤细胞可导致感染斑疹伤寒群立克次体（TGR）的靶细胞发生显著裂解，但对感染恙虫病立克次体的细胞无此作用。通过流式细胞术或对放射性标记的细胞表面蛋白进行免疫沉淀测定发现，针对TGR或感染TGR的细胞的单克隆和多克隆抗体不能特异性结合受感染的靶细胞。因此，在受感染靶细胞表面无法检测到TGR主要未改变的抗原决定簇。
