Gooding L R, O'Connell K A
J Immunol. 1983 Nov;131(5):2580-6.
Recognition by cytotoxic T lymphocytes (CTL) of syngeneic cells transformed with simian virus 40 (SV40) was examined by using cells expressing known portions of the SV40 early region both as sensitizing cells for stimulation of CTL development and as targets for lysis by CTL. LMTK- cells transfected with a plasmid specifying a 33K amino-terminal fragment of the SV40 tumor (T) antigen served both to stimulate syngeneic CTL capable of lysing SV40 transformed cells and as target cells for CTL generated against syngeneic transformants. The same is true for cells infected with the adenovirus 2-SV40 hybrid viruses, Ad2+ND1 and Ad2+ND2, which produce proteins homologous with carboxy-terminal regions of the SV40 T antigen. These findings indicate that on cells transformed by SV40 virus, and expressing the entire T protein, determinants in both the amino- and carboxy-terminal regions of the molecule are exposed on the cell surface. Furthermore, determinants in both these regions are recognized independently in the polyclonal CTL response to SV40.
通过使用表达猿猴病毒40（SV40）早期区域已知部分的细胞，作为刺激细胞毒性T淋巴细胞（CTL）发育的致敏细胞以及CTL裂解的靶细胞，来检测CTL对用SV40转化的同基因细胞的识别。用指定SV40肿瘤（T）抗原33K氨基末端片段的质粒转染的LMTK-细胞，既用于刺激能够裂解SV40转化细胞的同基因CTL，又作为针对同基因转化体产生的CTL的靶细胞。感染腺病毒2 - SV40杂交病毒Ad2 + ND1和Ad2 + ND2的细胞也是如此，它们产生与SV40 T抗原羧基末端区域同源的蛋白质。这些发现表明，在被SV40病毒转化并表达完整T蛋白的细胞上，该分子氨基末端和羧基末端区域的决定簇都暴露在细胞表面。此外，在对SV40的多克隆CTL反应中，这两个区域的决定簇是被独立识别的。
